RNA sequencing has revolutionized the exploration of gene expression. Advances in the sequencing workflow, from sample preparation through data analysis, enable rapid profiling and deep investigation of the transcriptome. With RNA sequencing, you can characterize all transcriptional activity, coding and non-coding, in any organism without a priori assumptions.

Illumina's unique combination of long and short reads, single and paired-end sequencing, strand specificity, and capacity for tens of millions to billions of reads per run allows you to...

• annotate coding SNPs
• discover transcript isoforms
• identify regulatory RNAs
• characterize splice junctions
• determine the relative abundance of transcripts

With the greatest daily output available for any sequencing system, transcript profiles can be generated in a single day. RNA sequencing reads can be aligned across splice junctions to identify isoforms, novel transcripts and gene fusions. Identify and quantify both rare and common transcripts, with over six orders of magnitude of dynamic range. Reveal the hidden world of non-coding RNA architecture without prior information.

Fully Supported Transcriptome Analysis Applications

• TruSeq RNA Sample Prep Kits: Illumina's latest high-throughput sample kits, providing robust indexing and a flexible, integrated workflow solution for economical RNA sequencing for discovery and profiling.
• mRNA-Seq: Illumina's original RNA sequencing sample prep kit providing the ability to prepare 8 libraries for full transcriptome analysis.
• TruSeq Small RNA Sample Prep Kit: Illumina's latest small RNA sample prep kit, with 48-sample indexing, enabling economical and high-throughput small RNA discovery and profiling.
• Small RNA Sample Prep: Illumina's original small RNA sequencing sample prep kit providing the ability to prepare 8 libraries for small RNA/microRNA analysis.