RNA sequencing has revolutionized the exploration of gene expression. Advances in the sequencing workflow, from sample preparation through data analysis, enable rapid profiling and deep investigation of the transcriptome. With RNA sequencing, you can characterize all transcriptional activity, coding and non-coding, in any organism without prior assumptions.

Illumina's unique combination of long and short reads, single and paired-end sequencing, strand specificity, and capacity for tens of millions to billions of reads per run allows you to...

• Annotate coding SNPs
• Discover transcript isoforms
• Identify regulatory RNAs
• Characterize splice junctions
• Determine the relative abundance of transcripts

With the greatest daily output available for any sequencing system, transcript profiles can be generated in a single day. RNA sequencing reads can be aligned across splice junctions to identify isoforms, novel transcripts and gene fusions. Identify and quantify both rare and common transcripts, with over six orders of magnitude of dynamic range.

Fully Supported Transcriptome Analysis Applications

• TruSeq Stranded Total RNA Sample Prep Kits: Coupled with Ribo-Zero rRNA removal chemistry, applies the power of TruSeq Stranded RNA sample preparation to whole-transcriptome analysis, capturing both mRNA and multiple forms of non-coding RNA in a highly scalable, end-to-end solution compatible with both normal and low-quality, FFPE samples.  
• TruSeq Stranded mRNA Sample Prep Kits: Provides the clearest, most complete mRNA-focused transcriptome analysis by providing precise measurement of strand orientation, uniform coverage, and high confidence mapping of alternate transcripts and gene fusion events. 
• TruSeq RNA Sample Prep Kits: RNA sample prep kits with robust indexing and a flexible, integrated workflow solution for economical RNA sequencing.
• TruSeq Small RNA Sample Prep Kit: Small RNA sample prep kit, with 48-sample indexing, enabling economical and high-throughput small RNA discovery and profiling.

New to RNA-Seq, or thinking about adding RNA-Seq to your workflow?

Go to the Getting Started Page to see why RNA-Seq delivers better data, workflow, and value.

Already using RNA-Seq?

Visit the Illumina RNA Resource Center for assay details, kits, literature, and protocols.

Sample Prep Kit Selection