Through the use of various fluorescent detection chemistries, Real-Time PCR (qPCR) enables the detection, amplification, and quantification of DNA sequences as a PCR reaction is proceeding. Unlike traditional PCR, samples are characterized at the point when amplification is first detected; as opposed to measuring the amount of product generated after PCR cycling is completed. This more precise and highly sensitive PCR method has applications in basic research and diagnostics, where it is used for gene expression and genotyping studies, and virus and pathogen detection.

Making qPCR Accessible

To achieve a higher level of detection, qPCR systems require powerful thermal and optical modules. For most qPCR systems, this translates into large instruments with price points to match. Making qPCR accessible to individual researchers, the compact Eco Real-Time PCR System packs loads of innovation into a small footprint that fits anywhere in the lab. A proprietary thermal system provides unrivaled temperature control and a sensitive optical system facilitates all four-color multiplex applications, enabling the system’s ability to deliver the performance, data quality, and reproducibility of market-leading instruments at a fraction of the price. This revolutionary new system supports a range of demanding Real-Time PCR applications, including absolute quantification by standard curve, relative quantification using the ∆∆Cq method with support for multiple reference gene normalization, allelic discrimination by end-point fluorescence, and gene scanning and genotyping by High Resolution Melt (HRM) curve analysis.