RNA sequencing has revolutionized the exploration of gene expression. Advances in the sequencing workflow, from sample preparation through data analysis, enable rapid profiling and deep investigation of the transcriptome. With the greatest daily output available for any sequencing system, transcript profiles can be generated in a single day. RNA sequencing reads can be aligned across splice junctions to identify isoforms, novel transcripts and gene fusions. Identify and quantify both rare and common transcripts, with over six orders of magnitude of dynamic range. With RNA sequencing, you can characterize all transcriptional activity, coding and non-coding, in any organism without prior assumptions.

New to RNA-Seq, or thinking about adding RNA-Seq to your workflow?

Go to the Getting Started Page to see why RNA-Seq delivers better data, workflow, and value.

Already using RNA-Seq?

Visit the Illumina RNA Resource Center for assay details, kits, literature, and protocols.

Fully Supported Transcriptome Analysis Applications

• TruSeq RNA Sample Prep Kits: Illumina's latest high-throughput sample kits, providing robust indexing and a flexible, integrated workflow solution for economical RNA sequencing for discovery and profiling.
• TruSeq Small RNA Sample Prep Kit: Illumina's latest small RNA sample prep kit, with 48-sample indexing, enabling economical and high-throughput small RNA discovery and profiling.
• Small RNA Sample Prep: Illumina's original small RNA sequencing sample prep kit providing the ability to prepare 8 libraries for small RNA/microRNA analysis.