Illumina TruSeq RNA Sample Preparation Kits provide a simple, cost-effective solution for generating libraries from total RNA. Master-mixed reagents eliminate the majority of pipetting steps and reduce the amount of clean-up, minimizing hands-on time. New automation-friendly workflow formats enable parallel processing of up to 96 samples. No specialized equipment is needed to prepare Illumina TruSeq RNA libraries, so any array lab can easily start the RNA-Seq workflow by preparing libraries with minimal investment and training.
The TruSeq RNA Sample Preparation Kit includes adapters containing unique index sequences that are ligated to sample fragments at the end of the library construction process. Because the indexed adapters are integrated into the basic workflow, every sample is tagged with a unique index sequence. This allows samples to be pooled for higher sequencing throughput, and then individually identified during downstream analysis.
Improved efficiencies in polyA selection and cDNA synthesis processes, including reduced sample transfers and removal of precipitation steps, eliminate the need for column purification and gel selection. This represents a 75% reduction in hands-on RNA-Seq workflow time vs earlier sample preparation kits for this portion of library construction. In addition, it allows for decreased input levels of RNA, as low as 100 ng of total RNA input.
The TruSeq Sample Preparation Kits are compatible with high-throughput, automated processing RNA-Seq workflows. Sample preparation can be performed in standard 96-well microplates with master-mixed reagents optimized for liquid-handling robots. Barcodes on reagents and plates allow end-to-end sample tracking and ensure that the correct reagents are used for the correct protocol, mitigating potential tracking errors.