Archival formalin-fixed paraffin-embedded (FFPE) samples hold an abundance of invaluable information for cancer studies. Because these samples generally yield highly degraded DNA, they typically perform poorly in array-based applications such as whole-genome genotyping. The Infinium HD FFPE DNA Restore Kit can repair these degraded DNA samples in preparation for use with the Infinium Assay.
After DNA extraction using one of many commercially available kits, the Illumina FFPE QC Kit uses a real-time PCR assay to evaluate the quality of prospective DNA samples. The assay utilizes standard instrumentation and reagents from an authorized vendor. Each kit provides primers and DNA template for 384 reactions.
Extracted FFPE samples that pass the QC test are eligible for restoration using the Infinium HD FFPE Restore Kit. The protocol restores degraded FFPE DNA to an amplifiable state.
Infinium FFPE QC and DNA Restoration Kits are designed to be used with Infinium BeadArray microarrays. The FFPE DNA Restore Kit is compatible with samples from any species, whereas the FFPE QC Kit is only compatible with human samples.
Analyzing FFPE Samples with Infinium Microarrays
Technical Note | PDF < 1 MB
FFPE DNA Restoration Solution Data Sheet
Data Sheet | PDF < 1 MB
Infinium HD FFPE QC Assay Protocol Guide Documentation
Infinium HD FFPE Lab Tracking Form (15021527) Documentation
Infinium HD FFPE Assay Reference Guide Documentation
Infinium HD FFPE Assay Auto Workflow Checklist Documentation
Infinium HD FFPE Assay Manual Workflow Checklist Documentation
Infinium FFPE QC and DNA Restoration Kit Support Documentation
Infinium Assay Consumables and Equipment List Documentation
Infinium HD FFPE Restore Protocol Guide (15014614) Documentation