TruSight One Sequencing Panels

Comprehensive sequencing research panels targeting disease-associated regions of the exome with high analytical sensitivity and specificity. Read More...
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Bundled Solutions

TruSight One Sequencing Combo (15 Samples)

20042621

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Enrichment Panels

TruSight One – Enrichment Oligos only (6 Enrichment Reactions)

20029227

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TruSight One Expanded – Enrichment Oligos only (6 Enrichment Reactions)

20029226

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Library Prep

Illumina® DNA Prep with Enrichment, (S) Tagmentation (16 Samples)

20025523

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Illumina® DNA Prep with Enrichment, (S) Tagmentation (96 Samples)

20025524

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Illumina® DNA Prep, (S) Tagmentation (16 Samples)

20025519

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Illumina® DNA Prep, (S) Tagmentation (96 Samples)

20025520

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Index Adapters

Illumina® DNA/RNA UD Indexes Set A, Tagmentation (96 Indexes, 96 Samples)

20091654

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Illumina® DNA/RNA UD Indexes Set B, Tagmentation (96 Indexes, 96 Samples)

20091656

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Illumina® DNA/RNA UD Indexes Set C, Tagmentation (96 Indexes, 96 Samples)

20091658

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Illumina® DNA/RNA UD Indexes Set D, Tagmentation (96 Indexes, 96 Samples)

20091660

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IDT® for Illumina® DNA/RNA UD Indexes Set A, Tagmentation (96 Indexes, 96 Samples)

20027213

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IDT® for Illumina® DNA/RNA UD Indexes Set B, Tagmentation (96 Indexes, 96 Samples)

20027214

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Product Highlights

TruSight One Sequencing Panels enable labs to expand and streamline their sequencing portfolio, while managing costs.

  • Two panels offer options to target up to 6700 genes associated with human disease, with high coverage
  • Single assay includes multiple genes of interest, replacing iterative testing
  • Easy, flexible gene filtering and annotation options simplify biological interpretation and reporting

These panels provide clinical research labs with an affordable solution for managing a diverse assay portfolio. The TruSight One Sequencing Panel provides comprehensive coverage of > 4800 disease-associated genes, while the TruSight One Expanded Sequencing Panel targets ~1900 additional genes with recent disease associations in the scientific literature.

Investigators can choose to analyze all of the genes on the panel or focus on a specific subset. With a single assay, labs can expand existing menus, streamline workflows, or create an entire portfolio of sequencing options.

Frequently Purchased Together

Plan on Using the TruSight One Enrichment Panel with NextSeq Reagents and Nextera Flex Enrichment?

Download this reference guide to understand which kit quantities you should purchase to support your research.

Download Reference Guide
Reference guide: TruSight One Enrichment Panel with NextSeq Reagents and Nextera Flex Enrichment

Specifications

Project Recommendations

Instrument Recommended Number of Samples Read Length
MiSeq System TruSight One: 3 samples per run with v3 reagents (based on 100x mean coverage of targeted content) Up to 2 × 150 bp
NextSeq 550 System TruSight One (samples per run): mid output: 12, high output: 36 (based on 100x mean coverage of targeted content)
TruSight One Expanded: 24 samples per run (high output; based on 100x mean coverage of targeted content)
Up to 2 × 150 bp
Up to 2 × 150 bp
NovaSeq 6000 System TruSight One Expanded: 96 samples per run (S1 flow cell; based on 100x mean coverage of targeted content) Up to 2 × 150 bp
NovaSeq 6000 System TruSight One Expanded: 96 samples per run (S1 flow cell; based on 100x mean coverage of targeted content) Up to 2 × 150 bp

Product Comparison

TruSight One Sequencing Panels TruSight Cardio Sequencing Panel
Content Specifications TruSight One: ~12 Mb genomic content (~4800 genes).
TruSight One Expanded: ~16.5 Mb genomic content (~6700 genes).
575 Kb genomic content (174 genes)
Description Fixed research panels targeting exonic regions that harbor disease-causing variants. Targeted sequencing research panel to identify causal variants associated with 17 inherited cardiac conditions.
Input Quantity 50 ng DNA 50 ng DNA
Method Target Enrichment, Target Enrichment, Targeted DNA Sequencing Target Enrichment, Target Enrichment, Targeted DNA Sequencing
Multiplexing Up to 96-plex Up to 96-plex
Specialized Sample Types Not FFPE-Compatible Not FFPE-Compatible
Species Category Human Human

Due to the larger size of the TruSight One Expanded Panel, Illumina recommends using a NextSeq, HiSeq, or NovaSeq system to achieve optimal sequencing coverage.

Method-Specific Workflow Example

 

Customer Stories

Gaining Insight into Genetic Disease

Dr. Kenjiro Kosaki furthers his clinical research and expands the genetic analysis services he provides with the TruSight One Sequencing Panel.

Read More

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