Bovine SNP50 Whole-Genome Genotyping Kits

BovineSNP50 DNA Analysis Kits

System: iScan, BeadStation, BeadArray Reader, HiScanSQ
Technology: BeadArray
Assay: Infinium DNA Analysis Assay
Applications: Focused Genotyping
Species: Bovine
Contents: Each package contains 4 to 96 BeadChips, along with reagents for amplifying, fragmenting, hybridizing, labeling, and detecting 48 to 1152 samples

The BovineSNP50 DNA Analysis BeadChip was developed by Illumina in collaboration with the USDA-ARS, University of Missouri, and the University of Alberta. The BeadChip contains 54,001 highly informative SNPs uniformly distributed across the entire genomes of major cattle breed types. All SNP probes on have been validated in 19 common beef and dairy breeds. This empowers applications such as genome-wide enabled selection, identification of quantitative trait loci, evaluation of genetic merit of individuals, and comparative genetic studies.

More than 24,000 SNP probes target novel SNP loci that were discovered by sequencing three pooled populations of economically important beef and dairy cattle using Illumina's Genome Analyzer IIx. Additional content is derived from publicly available sources such as the bovine reference genome, Btau1, and the Bovine HapMap Consortium data set. This product targets evenly distributed SNPs that are polymorphic across the breeds tested and provides an average probe spacing of 51.5kb and a median spacing of 37.3kb.

The BeadChip is a multi-sample genotyping panel powered by Illumina's Infinium HD Assay. This assay provides the industry's highest call rates, allows for flexible content deployment, and enables the detection and measurement of copy number variation.

  • This 12-sample BeadChip represents the highest density genotyping solution for characterizing the genome in dairy and beef cattle
  • The PCR-free, single tube sample preparation significantly reduces labor and potential sample handling errors

Supporting the most comprehensive genome-wide genotyping studies, the 777,000 SNP BovineHD BeadChip expands the diversity of bovine breeds assessed in genetic prediction and enables more discoveries of quantitative traits.

Order Information

WG-31-120
Bovine
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Required Products

Specifications

Product Specifications
               
BovineSNP50 v2 BeadChip  
Number of Markers 54,609
Samples per BeadChip 24
DNA Requiements 200 ng
Assay Infinium HD
Instrument iScan or HiScanSQ
Parameter Value From Reference Samples Product Specificaion
Call Rate 99.7% > 99%
Reproducibility 100% > 99.9%
Mendelian Inconsistencies 0.06% < 0.1%
 

 

 

Contents of the BovineSNP50 v2 whole-genome Genotyping kits:
  Catalog Number
WG-31-120
WG-31-121
WG-31-122
  
Number of Samples
48
288
1,152
Number of BeadChips
4
24
96
Ship at -65° to -85°C. Store at -15° to-25°C.
MA1 (Multi-Sample Amplification 1 Mix)
2.0 ml
12 ml (6 x 2.0 ml)
48 ml (24 x 2.0 ml)
MA2 (Multi-Sample Amplification 2 Mix)
3.9 ml
23.4 ml (6 x 3.9 ml)
93.6 ml (24 x 3.9 ml)
MSM (Multi-Sample Amplification Master Mix)
4.2 ml
25.2 ml (6 x 4.2 ml)
100.8 ml (24 x 3.0 ml)
FRG (Fragmentation Solution)
3.0 ml
18 ml (6 x 3.0 ml)
72 ml (24 x 4.2 ml)
XC1 (XStain BeadChip Solution 1)
2.0 ml
12 ml (6 x 2.0 ml)
48 ml (24 x 3.0 ml)
XC2 (XStain BeadChip Solution 2)
2.0 ml
12 ml (6 x 2.0 ml)
48 ml (24 x 2.0 ml)
TEM (Two-Color Extention Master Mix)
1.0 ml
6.0 ml (6 x 1.0 ml)
48 ml (24 x 2.0 ml)
LTM
(Labeling Two-Color Extension Master Mix)
3.3 ml
19.8 ml (6 x 3.3 ml)
24 ml (24 x 1.0 ml)
TEM
(Anti-Stain Two-Color Extension Master Mix)
2.3 ml
13.8 ml (6 x 2.3 ml)
55.2 ml (24 x 2.3 ml)
XC4 (XStain BeadChip Solution 4)
50 ml
150 ml (3 x 50 ml)
600 ml (12 x 50 ml)
RA1 (Resuspension,
Hybridization, and Wash Solution)
30 ml
90 ml (3 x 30 ml)
360 ml (12 x 30 ml)
E1BC (Wash Solution)
225 ml
675 ml (3 x 225 ml)
2.7 L (12 x 225 ml)
Ship at RT. Store at 2° to 8°C.
PA1 (Precipitation Solution)
7.0 ml
42 ml (6 x 7.0 ml)
168 ml (24 x 7.0 ml)
Ship at RT. Store at 2° to 8°C.
PB2 (Humidifying Buffer)
4.0 ml
12 ml (3 x 4.0 ml)
48 ml (12 x 4.0 ml)
XC4 (XStain BeadChip Solution 4)
80 ml
240 ml
960 ml (4 x 240 ml)
PB1 (Hybridization Preparation Buffer)
700 ml
2.1 L (3 x 700 ml)
8.4 L (12 x 700 ml)
 

Applications

Focused Genotyping

Focused genotyping supports a variety of applications such as candidate-gene studies in cancer, cardiovascular disease, and admixture mapping. Illumina also works closely with major animal consortia to develop genome-wide genotyping products for non-human organisms, including both animal and plant species.

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