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cBot Single Read Cluster Generation Kit
| System: | cBot, Genome Analyzer IIx |
|---|---|
| Technology: | Illumina Sequencing |
| Assay: | Single-Read Sequencing |
| Applications: | DNA Sequencing, Gene Regulation Analysis, SNP Discovery and Structural Variation Analysis, Cytogenetic Analysis, Sequencing-Based Methylation Analysis |
| Contents: | Reagents and consumables for processing one flow cell using the cBot with the Genome Analyzer IIx |
The cBot Single Read Cluster Generation Kit provides reagents that bind DNA library samples to complementary adapter oligonucleotides grafted on the flow cell surface. The pre-mixed, 96-well plate format reagents in this kit provide a streamlined workflow with no reagent preparation and a total of 10 minutes hands-on time.
Order Information
Required Products
Specifications
Learn more about how this product is used.
| Contents of the cbot single read cluster generation kit | |
|---|---|
| Catalog Number |
GD-300-1001 and GD-500-1001
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| Ship at -65° to -85°C. Store at -15° to -25°C. | |
| Row 1-HT1 (Hybridization Buffer) |
Note that all reagents are packaged in a sealed, 96-well plate
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| Row 2-HFE (1X Phusion TM Master Mix, Finnzymes Oy) |
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| Row 3-APM1 (AMX 1 Premix) |
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| Row 4-AMZ1 (Amplification Mix) |
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| Row 5-AT1 (100% Formamide) |
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| Row 6-AMX1 (Amplification Mix) |
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| Row 7-HT2 (Wash Buffer) |
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| Row 8-LS1 (Linearization Solution) |
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| Row 9-BMX (Blocking Mix) |
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| Row 10-HP5 (0.1 N NaOH) |
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| Row 11-HP1 (Sequencing Primer) |
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| Ship at RT. Store at RT. | |
| Single-Read Flow Cell |
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| Manifold |
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Applications
Sequencing
Illumina sequencing gives you the power to comprehensively characterize the genome, epigenome or the transcriptome. Go from DNA to data in under a week with less than four hours of hands-on time. Applications flexibility for any genetic variant. Superior raw-read accuracy and the industry's simplest, automated workflow. It's sequencing power for every researcher.
More...Gene Regulation Analysis
Get a complete view of the epigenome at single-base resolution with the Genome AnalyzerIIx. Whether you are interested in CpG methylation, histone modifications, chromatin structure, or DNA-protein interactions, Illumina's unique sequencing chemistry gives you unbiased coverage of every base in the genome.
More...SNP Discovery and Structural Variation Analysis
Single nucleotide polymorphisms (SNPs) and structural variants are at the root of genetic variation among individuals and populations. This variation influences how individuals differ in their risk of disease and their response to therapeutic treatments. SNPs and structural variants are discovered within the genome by comparing multiple genomic sequences from a diverse sample set of individuals. Illumina's industry-leading sequencing technology provides the sample throughput level and high-quality data required for accurate SNP discovery and structural variation analysis studies.
More...Cytogenetic Analysis
Structural variability is a substantial source of genetic variation that has a major influence on phenotypic variation. Cytogenetic analysis allows researchers to profile chromosomal aberrations such as amplifications, deletions, rearrangements, point mutations, copy number changes, and copy-neutral loss of heterozygosity (LOH) events.
More...Sequencing-Based Methylation Analysis
Identify and track methylation patterns by directly sequencing bisulfite-converted DNA, methylation-sensitive restriction digest-enriched fragments, anti-methyl C-precipitated fragments, or chromatin immunoprecipitates of methyltransferases trapped to aza-labeled DNA on the Illumina's portfolio of sequencing systems.
More...DNA Sequencing
Illumina’s sequencers powerfully combine the flexibility of single reads, short- and long-insert paired-end reads, enabling the broadest range of genomic applications. TruSeq sequencing chemistry supports this wide range of applications including whole-genome sequencing, targeted resequencing, de novo sequencing, SNP discovery, identification of copy number variations, and chromosomal rearrangement.
More...Documentation
Product Literature
At Illumina, our goal is to apply innovative technologies and revolutionary assays to the analysis of genetic variation and function, making studies possible that were not even imaginable just a few years ago. These studies will help make the realization of personalized medicine possible. With such rapid advances in technology taking place, it is mission critical to have solutions that are not only innovative, but flexible, scalable, and complete with industry-leading support and service. As a global company that places high value on collaborative interactions, rapid delivery of solutions, and prioritizing the needs of its customers, we strive to meet this challenge. Illumina's innovative, array-based solutions for DNA, RNA, and protein analysis serve as tools for disease research, drug development, and the development of molecular tests in the clinic.