ChIP-Seq DNA Sample Prep Kit ChIP-Seq DNA Sample Prep Workflow

ChIP-Seq DNA Sample Prep Workflow

ChIP-Seq DNA Sample Prep Kit

System: Genome Analyzer IIe, HiSeq 2000, HiScanSQ, cBot, Genome Analyzer IIx
Technology: Illumina Sequencing
Assay: Chromatin Immunoprecipitation Sequencing (ChIP-Seq)
Applications: DNA-Protein Interaction Analysis (ChIP-Seq)
Contents: Contains reagents for library preparation of chromatin-immunoprecipitated samples

Chromatin immunoprecipitation (ChIP) is a powerful method to selectively enrich for DNA sequences bound by a particular protein in living cells. ChIP-Seq on the Genome Analyzer supports virtually unconstrained selection of any ChIP-able protein and/or modification to be studied. These include transcription factors, polymerases and transcriptional machinery, structural proteins, protein modifications, and DNA modifications. The ChIP-Seq DNA Sample Prep Kit is used to build DNA libraries for single-read sequencing.

The ChIP process enriches specific crosslinked DNA protein complexes using an antibody against a protein of interest. Unique oligonucleotide adapters are then added to the small stretches of DNA that were bound to the protein of interest to enable massively parallel sequencing. Following a short PCR enrichment, the library is ready to load on cBot for cluster generation.

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IP-102-1001
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Contents of the Mate Pair Library Prep Kit

Applications

DNA-Protein Interaction Analysis (ChIP-Seq)

Accurately survey interactions between protein, DNA, and RNA to interpret regulation events central to many biological processes and disease states. Quantify in vivo protein-DNA interactions using the combination of chromatin immunoprecipitation with Illumina's sequencing technology (ChIP-Seq) on a genome-wide scale. Identify a broad range of interactions with confidence, and use millions of counts to differentiate real events from noise.

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