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ChIP-Seq DNA Sample Prep Kit
ChIP-Seq DNA Sample Prep Workflow
ChIP-Seq DNA Sample Prep Kit
| System: | Genome Analyzer, iScanSQ |
|---|---|
| Technology: | Illumina Sequencing |
| Assay: | Chromatin Immunoprecipitation Sequencing (ChIP-Seq) |
| Applications: | DNA-Protein Interaction Analysis (ChIP-Seq) |
| Contents: | Contains reagents for library preparation of chromatin-immunoprecipitated samples |
Chromatin immunoprecipitation (ChIP) is a powerful method to selectively enrich for DNA sequences bound by a particular protein in living cells. ChIP-Seq on the Genome Analyzer supports virtually unconstrained selection of any ChIP-able protein and/or modification to be studied. These include transcription factors, polymerases and transcriptional machinery, structural proteins, protein modifications, and DNA modifications. The ChIP-Seq DNA Sample Prep Kit is used to build DNA libraries for single-read sequencing.
The ChIP process enriches specific crosslinked DNA protein complexes using an antibody against a protein of interest. Unique oligonucleotide adapters are then added to the small stretches of DNA that were bound to the protein of interest to enable massively parallel sequencing. Following a short PCR enrichment, the library is ready to load on cBot for cluster generation.
- Use the ChIP-Seq Sample Prep Kit with a Standard Cluster Generation Kit and a 36-Cycle Illumina Sequencing Kit.
Applications
DNA-Protein Interaction Analysis (ChIP-Seq)
Accurately survey interactions between protein, DNA, and RNA to interpret regulation events central to many biological processes and disease states. Quantify in vivo protein-DNA interactions using the combination of chromatin immunoprecipitation with Illumina's sequencing technology (ChIP-Seq) on a genome-wide scale. Identify a broad range of interactions with confidence, and use millions of counts to differentiate real events from noise.
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