ChIP-Seq DNA Sample Prep Workflow
| System: | Genome Analyzer, iScanSQ |
|---|---|
| Technology: | Illumina Sequencing |
| Assay: | Chromatin Immunoprecipitation Sequencing (ChIP-Seq) |
| Applications: | DNA-Protein Interaction Analysis (ChIP-Seq) |
| Contents: | Contains reagents for library preparation of chromatin-immunoprecipitated samples |
Chromatin immunoprecipitation (ChIP) is a powerful method to selectively enrich for DNA sequences bound by a particular protein in living cells. ChIP-Seq on the Genome Analyzer supports virtually unconstrained selection of any ChIP-able protein and/or modification to be studied. These include transcription factors, polymerases and transcriptional machinery, structural proteins, protein modifications, and DNA modifications. The ChIP-Seq DNA Sample Prep Kit is used to build DNA libraries for single-read sequencing.
The ChIP process enriches specific crosslinked DNA protein complexes using an antibody against a protein of interest. Unique oligonucleotide adapters are then added to the small stretches of DNA that were bound to the protein of interest to enable massively parallel sequencing. Following a short PCR enrichment, the library is ready to load on cBot for cluster generation.
Accurately survey interactions between protein, DNA, and RNA to interpret regulation events central to many biological processes and disease states. Quantify in vivo protein-DNA interactions using the combination of chromatin immunoprecipitation with Illumina's sequencing technology (ChIP-Seq) on a genome-wide scale. Identify a broad range of interactions with confidence, and use millions of counts to differentiate real events from noise.
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