Chromatin immunoprecipitation (ChIP) selectively finds DNA sequences bound by a particular protein. The ChIP-Seq DNA Sample Prep Kit helps build DNA libraries. The ChIP process enriches specific crosslinked DNA protein complexes using an antibody and unique oligonucleotide adapters added to small stretches of DNA bound to the protein of interest.

Chromatin immunoprecipitation (ChIP) is a powerful method to selectively enrich for DNA sequences bound by a particular protein in living cells. ChIP-Seq on Illumina sequencing systems supports virtually unconstrained selection of any ChIP-able protein and/or modification to be studied. These include transcription factors, polymerases and transcriptional machinery, structural proteins, protein modifications, and DNA modifications. This kit is used to build DNA libraries for single-read sequencing.

The ChIP process enriches specific crosslinked DNA protein complexes using an antibody against a protein of interest. Unique oligonucleotide adapters are then added to the small stretches of DNA that were bound to the protein of interest to enable massively parallel sequencing. Following a short PCR enrichment, the library is ready to load on cBot or Cluster Station for cluster generation.

System Compatibility

HiScanSQ
HiSeq 1000
HiSeq 2000
HiSeq 2500
NextSeq 500 Sequencing System

Catalog IDs: IP-102-1001

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