Custom GoldenGate Panels (OPAs)

Illumina Custom GoldenGate Genotyping Panels allow researchers to create assays tailored directly to their specific needs for targeted region genotyping or fine-mapping of candidate disease association regions. GoldenGate Custom Panels provide flexibility and high data quality across a wide range of genomes and experimental designs. They are available in panels of 96 assays and multiples of 96, from 384-1,536, assays per oligo pool assay (OPA) tube; larger panels can be accommodated with multiple OPA tubes.

Customers can design custom GoldenGate Assays to target specific SNP loci for their organism of study. Illumina scientists and a proprietary Assay Design Tool (ADT) help customers create successful content panels. The ADT provides a simple and powerful method for evaluating individual loci and creating the most successful custom genotyping assays.

Researchers create custom panels by selecting and submitting a requested list of loci to Illumina, which is evaluated with the ADT. Custom content designs can be submitted to a web application using sequence, accession number, gene symbol, gene ID, GI number, or chromosomal region. The SNPScore file provides predicted success information, validation status, and minor allele frequencies from published studies. Researchers use the ADT SNPScore file output to refine an initial assay panel to include desired assays that are predicted to have a high likelihood of success. This information can be used to reject some SNPs and ultimately create a final SNPScore file for the order.

• The GoldenGate Assay may be deployed on BeadArray or BeadXpress platforms

• The BeadArray platform supports the highest multiplex levels, while the BeadXpress platform is an ideal option for high-throughput biomarker screening