Tag Profiling Cluster Generation Kits
| System: | Genome Analyzer IIe |
|---|---|
| Technology: | Illumina Sequencing |
| Applications: | Sequencing-Based Transcriptome Analysis |
| Contents: | Contains reagents, flow cell(s), amplification manifold, and hybridization manifold for processing samples. NlaIII and DpnII kits are separate |
Please note that this product is in the process of being discontinued. Available while supplies last or until Spring 2010, whichever comes first.
Tag Profiling Cluster Generation Kits are used with total RNA samples to bind the tag construct library to complementary adapter oligos grafted on the flow cell surface. Cluster Station is used to isothermally amplify the attached cDNA constructs to create clonal clusters of ~ 1,000 copies each.
Tag Profiling Sample Prep Kits are used to build constructs comprised of a unique, positionally known 20- or 21-base pair cDNA tag with defined adapters attached to both ends. Access to a vast majority of messenger RNAs is provided through two restriction enzyme tag construction options.
Order Information
Required Products
Specifications
| Contents of the DGE: tag profiling cluster generation kits: | |
|---|---|
| Box 1 |
Nla lll-based Kits
Dpn ll-based Kits
|
| Cluster Buffer |
7.5 ml
7.5 ml
|
| Bst DNA Polymerase |
380 µl
380 µl
|
| 10 mM dNTPs |
750 µl
750 µl
|
| 1M Tris pH 8 |
65 µl
65 µl
|
| ddNTPs (130 µM) |
35 µl
35 µl
|
| Terminal Transferase |
25 µl
25 µl
|
| Blocking Buffer |
350 µl
350 µl
|
| Taq DNA Polymerase |
30 µl
30 µl
|
| Box 2 |
|
| Hybridization Buffer |
12 ml
12 ml
|
| Wash Buffer |
10 ml
10 ml
|
| Ultrapure Water |
50 ml
50 ml
|
| 0.1N NaOH |
1.5 ml
1.5 ml
|
| Sodium Periodate |
68 mg
68 mg
|
| 2N NaOH |
20 µl
20 µl
|
| TE |
1.5 ml
1.5 ml
|
| Formamide |
35 ml
35 ml
|
| Storage Buffer |
50 ml
50 ml
|
| Flow Cells |
1 per 8 samples
1 per 8 samples
|
| Bag 1 |
|
| Nla lll Sequencing Primer |
10 µl
n/a
|
| Dpn ll Sequencing Primer |
n/a
10 µl
|
| Shipping and Storage Conditions: | |||
|---|---|---|---|
|
Shipping Temperature
Storage Temperature
|
|||
| Box 1 |
-80°C
-20°C
|
||
| Box 2 |
RT
RT
|
||
| Box 1 |
-80°C
-20°C
|
||
Applications
Transcriptome Analysis
Explore the transcriptome like never before. Characterize all transcriptional activity, coding and non-coding, in any organism without a priori assumptions using the Genome Analyzer and achieve an unprecedented view of the transcriptome.
Illumina's unique combination of long and short reads, strand specificity, and millions of counts allows you to annotate coding SNPs, discover transcript isoforms, identify regulatory RNAs, characterize splice junctions, and determine the relative abundance of transcripts.
Generate transcript profiles in a single day with the greatest daily system output. Align RNA sequencing reads across splice junctions and map to reference genomes. Identify and quantify both rare and common transcripts, with six orders of magnitude of dynamic range. Reveal the hidden world of non-coding RNA architecture without prior information.
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