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Tag Profiling Cluster Generation Kits
Tag Profiling Cluster Generation Kits
| System: | Genome Analyzer |
|---|---|
| Technology: | Illumina Sequencing |
| Applications: | Sequencing-Based Transcriptome Analysis |
| Contents: | Contains reagents, flow cell(s), amplification manifold, and hybridization manifold for processing samples. NlaIII and DpnII kits are separate |
Please note that this product is in the process of being discontinued. Available while supplies last or until Spring 2010, whichever comes first.
Tag Profiling Cluster Generation Kits are used with total RNA samples to bind the tag construct library to complementary adapter oligos grafted on the flow cell surface. Cluster Station is used to isothermally amplify the attached cDNA constructs to create clonal clusters of ~ 1,000 copies each.
Tag Profiling Sample Prep Kits are used to build constructs comprised of a unique, positionally known 20- or 21-base pair cDNA tag with defined adapters attached to both ends. Access to a vast majority of messenger RNAs is provided through two restriction enzyme tag construction options.
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Transcriptome Analysis
Explore the transcriptome like never before. Characterize all transcriptional activity, coding and non-coding, in any organism without a priori assumptions using the Genome Analyzer and achieve an unprecedented view of the transcriptome.
Illumina's unique combination of long and short reads, strand specificity, and millions of counts allows you to annotate coding SNPs, discover transcript isoforms, identify regulatory RNAs, characterize splice junctions, and determine the relative abundance of transcripts.
Generate transcript profiles in a single day with the greatest daily system output. Align RNA sequencing reads across splice junctions and map to reference genomes. Identify and quantify both rare and common transcripts, with six orders of magnitude of dynamic range. Reveal the hidden world of non-coding RNA architecture without prior information.
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