Tag Profiling Sample Prep Kits
| System: | HiSeq 2000, HiScanSQ, cBot, Genome Analyzer IIx, HiSeq 1000 |
|---|---|
| Technology: | Illumina Sequencing |
| Contents: | Contains reagents for preparing NIaIII or DpnII tags from total RNA samples. Oligo only kits contain only the required adapters and PCR Primers for NIaIII tag or DpnII tag formation |
Please note that this product is in the process of being discontinued. Available while supplies last or until Spring 2010, whichever comes first.
Tag Profiling Sample Prep Kits are used to build constructs comprised of a unique, positionally known 20- or 21-base pair cDNA tag with defined adapters attached to both ends. Access to a vast majority of messenger RNAs is provided through two restriction enzyme tag construction options.
Tag Profiling Sample Prep Kits are available as complete validated kits or as oligo-only kits containing only the oligonucleotides required for the protocol.
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Transcriptome Analysis
RNA sequencing has revolutionized the exploration of gene expression. Advances in the sequencing workflow, from sample preparation through data analysis, enable rapid profiling and deep investigation of the transcriptome. With RNA sequencing, you can characterize all transcriptional activity, coding and non-coding, in any organism without a priori assumptions.
Illumina's unique combination of long and short reads, single and paired-end sequencing, strand specificity, and capacity for tens of millions to billions of reads per run allows you to- annotate coding SNPs
- discover transcript isoforms
- identify regulatory RNAs
- characterize splice junctions
- determine the relative abundance of transcripts
With the greatest daily output available for any sequencing system, transcript profiles can be generated in a single day. RNA sequencing reads can be aligned across splice junctions to identify isoforms, novel transcripts and gene fusions. Identify and quantify both rare and common transcripts, with over six orders of magnitude of dynamic range. Reveal the hidden world of non-coding RNA architecture without prior information.
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