Products /
Tag Profiling Sample Prep Kits
Tag Profiling Sample Prep Kits
| System: | Genome Analyzer |
|---|---|
| Technology: | Illumina Sequencing |
| Contents: | Contains reagents for preparing NIaIII or DpnII tags from total RNA samples. Oligo only kits contain only the required adapters and PCR Primers for NIaIII tag or DpnII tag formation |
Please note that this product is in the process of being discontinued. Available while supplies last or until Spring 2010, whichever comes first.
Tag Profiling Sample Prep Kits are used to build constructs comprised of a unique, positionally known 20- or 21-base pair cDNA tag with defined adapters attached to both ends. Access to a vast majority of messenger RNAs is provided through two restriction enzyme tag construction options.
Tag Profiling Sample Prep Kits are available as complete validated kits or as oligo-only kits containing only the oligonucleotides required for the protocol.
- Tag Profiling requires the use of a Tag Profiling Sample Prep Kit, a Tag Profiling Cluster Generation Kit, and an 18-Cycle Illumina Sequencing Kit
Specifications
Applications
Transcriptome Analysis
Explore the transcriptome like never before. Characterize all transcriptional activity, coding and non-coding, in any organism without a priori assumptions using the Genome Analyzer and achieve an unprecedented view of the transcriptome.
Illumina's unique combination of long and short reads, strand specificity, and millions of counts allows you to annotate coding SNPs, discover transcript isoforms, identify regulatory RNAs, characterize splice junctions, and determine the relative abundance of transcripts.
Generate transcript profiles in a single day with the greatest daily system output. Align RNA sequencing reads across splice junctions and map to reference genomes. Identify and quantify both rare and common transcripts, with six orders of magnitude of dynamic range. Reveal the hidden world of non-coding RNA architecture without prior information.
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