Equine SNP50 DNA Analysis Kits
| Contents: | Each package contains 4 to 96 BeadChips, along with reagents for amplifying, fragmenting, hybridizing, labeling, and detecting 48 to 1152 samples |
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The Equine SNP50 BeadChip was developed through the Illumina iSelect program in collaboration with the International Equine Genome Mapping Workshop and the Morris Animal Foundation's Equine Genome Consortium. The BeadChip features 54,602 highly informative SNPs uniformly distributed across the entire genomes of 15 breeds of horses.
Content is derived from the EquCab2.0 SNP collection compiled by the Broad Institute's Equine Genome Sequencing Project. The researchers discovered more than 940,000 SNPs across Arabian, Andalusian, Akhal-teke, Icelandic, Standardbred, Thoroughbred, and Quarter horse breeds. From this data set, the EquineSNP50 BeadChip developers designed probes that target a subset of uniformly distributed SNPs spanning the entire horse genome.
With an average probe spacing of 43.2kb across all 31 autosomes, the EquineSNP50 BeadChip provides more than sufficient marker information to support whole-genome enabled selection. Over 53,500 probes target polymorphic loci, including 2,254 loci that appear to have an adjacent or third polymorphism such as a deletion or third allele.
This BeadChip enables horse researchers to conduct a broad range of genotyping applications, including genome-wide association studies and Quantitative Trait Loci identification. Designed to enable identification of genes and mutations that contribute to traits of interest in all major horse breeds, this BeadChip offers a powerful platform for improving horse breeding programs. More, it enables the development of new diagnostic and therapeutic approaches to promote equine health and welfare.
Order Information
Required Products
Specifications
Learn more about how this product is used.
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Product Specifications
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| EquineSNP50 | |||||||
| Number of Markers | 54,602 | ||||||
| Samples per BeadChip | 12 | ||||||
| DNA Requiements | 200 ng | ||||||
| Assay | Infinium HD | ||||||
| Instrument | iScan or HiScan | ||||||
| Parameter | Value From Reference Samples | Product Specificaion | |||||
| Call Rate | 99.54% | > 99% | |||||
| Mendelian Inconsistencies | 0.01% | < 0.1% | |||||
| Reproducibility | 100% | < 99.9% | |||||
* Across the 14 breeds genotyped. |
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| Contents of the EquineSNP50 whole-genome Genotyping kits: | |
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| Catalog Number |
WG-402-1001
WG-402-1002
WG-402-1003
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| Number of Samples |
48
288
1,152
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| Number of BeadChips |
4
24
96
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| Ship at -65° to -85°C. Store at -15° to -25°C. | |
| MA1 (Multi-Sample Amplification 1 Mix) |
2.0 ml
12 ml (6 x 2.0 ml)
48 ml (24 x 2.0 ml)
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| MA2 (Multi-Sample Amplification 2 Mix) |
3.9 ml
23.4 ml (6 x 3.9 ml)
93.6 ml (24 x 3.9 ml)
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| MSM (Multi-Sample Amplification Master Mix) |
4.2 ml
25.2 ml (6 x 4.2 ml)
100.8 ml (24 x 4.2 ml)
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| FRG (Fragmentation Solution) |
3.0 ml
18 ml (6 x 3.0 ml)
72 ml (24 x 3.0 ml)
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| XC1 (XStain BeadChip Solution 1) |
2.0 ml
12 ml (6 x 2.0 ml)
48 ml (24 x 3.0 ml)
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| XC2 (XStain BeadChip Solution 2) |
2.0 ml
12 ml (6 x 2.0 ml)
48 ml (24 x 2.0 ml)
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| TEM (Two-Color Extention Master Mix) |
1.0 ml
6.0 ml (6 x 1.0 ml)
24 ml (24 x 1.0 ml)
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| LEM (Labeling Two-Color Extension Master Mix) |
3.3 ml
19.8 ml (6 x 3.3 ml)
79.2 ml (24 x 3.3 ml)
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| ATM (Anti-Stain Two-Color Extension Master Mix) |
2.3 ml
13.8 ml (6 x 2.3 ml)
55.2 ml (24 x 2.3 ml)
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| XC4 (XStain BeadChip Solution 4) |
50 ml
150 ml (3 x 50 ml)
600 ml (12 x 50 ml)
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| RA1 (Resuspension, Hybridization, and Wash Solution) |
30 ml
90 ml (3 x 30 ml)
360 ml (12 x 30 ml)
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| E1BC (Wash Solution) |
225 ml
675 ml (3 x 225 ml)
2.7 L (12 x 225 ml)
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| Ship at RT. Store at 2° to 8°C. | |
| PA1 (Precipitation Solution) |
7.0 ml
42 ml (6 x 7.0 ml)
168 ml (24 x 7.0 ml)
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| Ship at RT. Store at RT. | |
| PB2 (Humidifying Buffer) |
4.0 ml
12 ml (3 x 4.0 ml)
48 ml (12 x 4.0 ml)
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| XC3 (XStain BeadChip Solution 3) |
80 ml
240 ml
960 ml (4 x 240 ml)
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| PB1 (Hybridization Preparation Buffer) |
700 ml
2.1 L (3 x 700 ml)
8.4 L (12 x 700 ml)
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Applications
Focused Genotyping
Focused genotyping supports a variety of applications such as candidate-gene studies in cancer, cardiovascular disease, and admixture mapping. Illumina also works closely with major animal consortia to develop genome-wide genotyping products for non-human organisms, including both animal and plant species.
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