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HiSeq Sequencing Kit (200 cycles)
HiSeq Sequencing Kit (200 cycles)
| System: | HiSeq 2000 |
|---|---|
| Technology: | Illumina Sequencing |
| Assay: | Single-Read Sequencing, mRNA-Seq, Mate-Pair Library Sequencing, Multiplex Sequencing, Paired-End Sequencing |
| Applications: | DNA Sequencing, Gene Regulation Analysis, SNP Discovery and Structural Variation Analysis, Cytogenetic Analysis, Sequencing-Based Methylation Analysis |
| Contents: | Provides kitted reagents for up to 209 cycles of sequencing on the HiSeq 2000 sequencing system |
The HiSeq 2000 sequencing system requires the HiSeq Sequencing Kit.
Pre-configured reagents reduce hands-on preparation time to two minutes. Sequencing by synthesis (SBS) technology takes advantage of proprietary, fluorescently-labeled, reversibly terminated nucleotides to sequence the hundreds of millions of clusters, base by base, in parallel.
The HiSeq Sequencing Kit (200 cycles) is used for:
- Single-Read Sequencing
- Paired-End Sequencing
- Mate-Pair Library Sequencing
- Multiplex Sequencing
- ChIP-Seq Sequencing
- mRNA-Seq
- Small RNA Discovery and Analysis
Note: The HiSeq Sequencing Kit (200 cycles) is NOT compatible with the Genome Analyzer.
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Applications
Sequencing
Illumina sequencing gives you the power to comprehensively characterize the genome, epigenome or the transcriptome. Go from DNA to data in under a week with less than four hours of hands-on time. Applications flexibility for any genetic variant. Superior raw-read accuracy and the industry’s simplest, automated workflow. It’s sequencing power for every researcher.
More...Gene Regulation Analysis
Get a complete view of the epigenome at single-base resolution with the Genome Analyzer. Whether you are interested in CpG methylation, histone modifications, chromatin structure, or DNA-protein interactions, Illumina's unique sequencing chemistry gives you unbiased coverage of every base in the genome.
More...SNP Discovery and Structural Variation Analysis
Single nucleotide polymorphisms (SNPs) and structural variants are at the root of genetic variation among individuals and populations. This variation influences how individuals differ in their risk of disease and their response to therapeutic treatments. SNPs and structural variants are discovered within the genome by comparing multiple genomic sequences from a diverse sample set of individuals. Illumina's industry-leading sequencing technology provides the sample throughput level and high-quality data required for accurate SNP discovery and structural variation analysis studies.
More...Sequencing-Based Methylation Analysis
Identify and track methylation patterns by directly sequencing bisulfite-converted DNA, methylation-sensitive restriction digest-enriched fragments, anti-methyl C-precipitated fragments, or chromatin immunoprecipitates of methyltransferases trapped to aza-labeled DNA on the Genome Analyzer.
More...Cytogenetic Analysis
Structural variability is a substantial source of genetic variation that has a major influence on phenotypic variation. Cytogenetic analysis allows researchers to profile chromosomal aberrations such as amplifications, deletions, rearrangements, point mutations, copy number changes, and copy-neutral loss of heterozygosity (LOH) events.
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