HiSeq Single-Read Cluster Generation Kit
| System: | HiSeq 2000, HiScanSQ, cBot, HiSeq 1000 |
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| Technology: | Illumina Sequencing |
| Assay: | Single-Read Sequencing, mRNA-Seq, Small RNA Discovery and Analysis, Multiplex Sequencing |
| Applications: | DNA Sequencing, Gene Regulation Analysis, SNP Discovery and Structural Variation Analysis, Cytogenetic Analysis, Sequencing-Based Methylation Analysis |
| Contents: | Provides kitted reagents for cluster generation on 1 single-read flow cell for the HiSeq and HiScanSQ sequencing systems |
The HiSeq Single-Read Cluster Generation Kit provides reagents for cluster amplification on single-read flow cells using the cBot cluster amplification system. cBot cluster generation reagents are provided in a pre-mixed, 96-well plate format that requires no reagent preparation.
Note: This Cluster Generation Kit is used for preparing sequencing templates for HiSeq 2000, HiSeq 1000, and HiScanSQ. It is not compatible with the Genome Analyzer.
DNA library samples are bound to complementary adapter oligos grafted on the surface of the Illumina sequencing flow cell. The templates are copied from the hybridized primer by 3' extension using a high fidelity DNA polymerase to prevent misincorporation errors. These copies are isothermally amplified to create clonal clusters of ~1,000 copies each, ready for sequencing.
Order Information
Specifications
Learn more about how this product is used.
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Contents of the HiSeq SR Cluster Generation Kit
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| Catalog Number | GD-401-1001 | ||||||
| HiSeq Cluster Generation Reagent Plate Ship on dry ice. Store at -15° to -25°C. | |||||||
| HT1 (Hybridization Buffer) | |||||||
| HFE (1x PhusionTM Master Mix (Finnzymes Oy)) |
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| APM1 (AMX 1 Premix) | |||||||
| AMX1 (Amplification Mix) | |||||||
| AT1 (100% Formamide) | |||||||
| AMX1 (Amplification Mix) | |||||||
| HT2 (Wash Buffer) | |||||||
| LS1 (Linearization Solution) | |||||||
| BMX (Blocking Mix) | |||||||
| HP5 (0.1 N NaOH) | |||||||
| HP1 (Sequencing Primer) | |||||||
| Single-Read HiSeq Flow Cell Ship at RT. Store at 2°C to 8°C. |
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| Manifold & HiSeq Accessories Kit Shap at RT. Store at RT. |
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Contents of the HiSeq Sequencing Kit
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| Catalog Number | FC-401-1001 | ||||||
| Box 1 Ship on dry ice. Store at -15° to -20°C. |
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| CMR (Cleavage Mix Reagent) | |||||||
| SMR (Scanning Mix Reagent) | |||||||
| LRP (Long Read DNA Polymerase) | |||||||
| LFN36 (Long Read Nucleotide Mix) | |||||||
| Box 2 Ship on dry ice. Store at 2° to 8°C. |
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| PW1 (Wash Solution) | |||||||
| ICR (Incorporation Mix Reagent) | |||||||
| SB1 (High Salt Buffer) | |||||||
| SB2 (Incorporation Buffer) | |||||||
| SB3 (Cleavage Buffer) | |||||||
Applications
Sequencing
Illumina sequencing gives you the power to comprehensively characterize the genome, epigenome or the transcriptome. Go from DNA to data in under a week with less than four hours of hands-on time. Applications flexibility for any genetic variant. Superior raw-read accuracy and the industry's simplest, automated workflow. It's sequencing power for every researcher.
More...Gene Regulation Analysis
Get a complete view of the epigenome at single-base resolution with the Genome AnalyzerIIx. Whether you are interested in CpG methylation, histone modifications, chromatin structure, or DNA-protein interactions, Illumina's unique sequencing chemistry gives you unbiased coverage of every base in the genome.
More...SNP Discovery and Structural Variation Analysis
Single nucleotide polymorphisms (SNPs) and structural variants are at the root of genetic variation among individuals and populations. This variation influences how individuals differ in their risk of disease and their response to therapeutic treatments. SNPs and structural variants are discovered within the genome by comparing multiple genomic sequences from a diverse sample set of individuals. Illumina's industry-leading sequencing technology provides the sample throughput level and high-quality data required for accurate SNP discovery and structural variation analysis studies.
More...Sequencing-Based Methylation Analysis
Identify and track methylation patterns by directly sequencing bisulfite-converted DNA, methylation-sensitive restriction digest-enriched fragments, anti-methyl C-precipitated fragments, or chromatin immunoprecipitates of methyltransferases trapped to aza-labeled DNA on the Genome Analyzer IIx.
More...Cytogenetic Analysis
Structural variability is a substantial source of genetic variation that has a major influence on phenotypic variation. Cytogenetic analysis allows researchers to profile chromosomal aberrations such as amplifications, deletions, rearrangements, point mutations, copy number changes, and copy-neutral loss of heterozygosity (LOH) events.
More...
