HumanCVD Whole-Genome Genotyping Kits
| Contents: | Each kits contains 4 to 96 BeadChips, along with reagents for amplifying, fragmenting, hybridizing, labeling, and detecting 48 to 1152 samples |
|---|
The HumanCVD BeadChip is the first high-density single nucleotide polymorphism (SNP) genotyping standard panel specifically targeted for cardiovascular disease (CVD) studies. The BeadChip features nearly 50,000 markers that capture genetic diversity across approximately 2,100 genes associated with CVD processes such as blood pressure changes, insulin resistance, metabolic disorders, dyslipidemia, and inflammation.
The content of this BeadChip was validated using 117 HapMap samples and targets regions associated with a range of cardiovascular, metabolic, and inflammatory syndromes, which can be divided into three broad groups:
- Group 1 loci comprise approximately 435 genes and regions with a high likelihood of functional significance. Tag SNPs derived from the HapMap Project and SeattleSNPs data sets were selected to capture all known variants in these regions
- Group 2 loci, also based on HapMap Project and SeattleSNPs data, are a set of 1,349 loci potentially involved in cardiovascular pathologies and other metabolic, heart, lung, blood, or circadian phenotypes
- Group 3 loci comprise approximately 232 larger genes which were of lower interest to the CVD consortium investigators. Only nonsynonymous SNPs and known functional variants were included from these regions
The HumanCVD BeadChip also contains more than 1,500 admixture and ancestry informative SNP markers (AIMs) for African versus European ancestry, and it features 400 AIMs for regional European ancestral populations.
This BeadChip was developed in collaboration with researchers from the Institute of Translational Medicine and Therapeutics, the Broad Institute, SeattleSNPs, and the Candidate-gene Association Resource (CARe).
The HumanCVD BeadChip is a multi-sample genotyping panel powered by Illumina’s Infinium Assay. This revolutionary assay provides the industry’s highest call rates, allows for flexible content deployment, and enables the detection and measurement of copy number variation.
Order Information
Required Products
Specifications
Learn more about how this product is used.
|
Product Specifications
|
|||||||
|---|---|---|---|---|---|---|---|
| HumanCVD | |||||||
| Number of Markers | 48,742 | ||||||
| Samples per BeadChip | 12 | ||||||
| DNA Requiements | 200 ng | ||||||
| Assay | Infinium HD | ||||||
| Instrument | iScan or HiScanSQ | ||||||
| Parameter | Value From Reference Samples | Product Specificaion | |||||
| Call Rate | >99% | > 99.79% | |||||
| Reproducibility | >99% | > 99.99% | |||||
| Mendelian Inconsistencies | <0.01% | < 0.001% | |||||
|
|
|||||||
| Contents of the HumanCVD whole-genome Genotyping kits: | |
|---|---|
| Catalog Number |
WG-31-151
WG-31-152
WG-31-153
|
| Number of Samples |
48
288
1,152
|
| Number of BeadChips |
4
24
96
|
| Ship at -65° to -85°C. Store at -15° to -25°C. | |
| MP1 (Netralization Solution) |
2.5 ml
7.5 ml (3 x 2.5 ml)
30 ml (12 x 2.5 ml)
|
| AMM (Amplification Master Mix) |
2.7 ml
8.1 ml (3 x 2.7 ml)
32.4 ml (12 x 2.7 ml)
|
| FRG (Fragmentation Solution) |
1.9 ml
5.7 ml (3 x 1.9 ml)
22.8 ml (12 x 1.9 ml)
|
| RA1 (Resuspension, Hybridization, and Wash Solution) |
30 ml
90 ml (3 x 30 ml)
360 ml (12 x 30 ml)
|
| XC1 (XStain BeadChip Solution 1) |
4 ml (2 x 2 ml)
12 ml (6 x 2 ml)
48 ml (24 x 2 ml)
|
| XC2 (XStain BeadChip Solution 2) |
4 ml (2 x 2 ml)
12 ml (6 x 2 ml)
48 ml (24 x 2 ml)
|
| XC4 (XStain BeadChip Solution 4) |
29 ml
87 ml (3 x 29 ml)
248 ml (12 x 29 ml)
|
| TEM (Two-Color Extention Master Mix) |
2 ml (2 x 1 ml)
6 ml (6 x 1 ml)
24 ml (24 x 1 ml)
|
| LTM (Labeling Two-Color Extension Master Mix) |
6.6 ml (2 x 3.3 ml)
19.8 ml (6 x 3.3 ml)
79.2 ml (24 x 3.3 ml)
|
| ATM (Anti-Stain Two-Color Master Mix) |
4.6 ml (2 x 2.3 ml)
13.8 ml (6 x 2.3 ml)
55.2 ml (246 x 2.3 ml)
|
| Ship at RT. Store at 2° to 8°C. | |
| PA1 (Precipitation Solution) |
5 ml
15 ml (3 x 5 ml)
60 ml (12 x 5 ml)
|
| Ship at -65° to -85°C. Store at RT. (Note: Ships at RT for 1, 152-sample kit.) | |
| PB1 (Hybridization Preparation Buffer) |
700 ml
2,100 ml (3 x 700 ml)
8,400 ml (12 x 700 ml)
|
| PB2 (Humidifying Buffer) |
4 ml
12 ml (3 x 4 ml)
48 ml (12 x 4 ml)
|
| Ship at -65° to -85°C. Store at RT. (Note: Ships at RT for 1, 152-sample kit.) | |
| XC3 (XStain BeadChip Solution 3) |
80 ml
240 ml
960 ml (4 x 2400 ml)
|
Applications
Focused Genotyping
Focused genotyping supports a variety of applications such as candidate-gene studies in cancer, cardiovascular disease, and admixture mapping. Illumina also works closely with major animal consortia to develop genome-wide genotyping products for non-human organisms, including both animal and plant species.
More...
