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HumanHT-12 v3 Expression BeadChip Kits
HumanHT-12 v3 Expression BeadChip Kits
| System: | iScan, iScanSQ, BeadArray Reader |
|---|---|
| Technology: | BeadArray |
| Assay: | Direct Hybridization Assay |
| Applications: | Whole-Genome Gene Expression Analysis |
| Species: | Human |
| Contents: | Each kit includes 2-6 BeadChips plus reagents for hybridization, washing, and processing RNA samples |
The HumanHT-12 Expression BeadChip contains the same panel of probes as the HumanWG-6 BeadChip, but provides a higher throughput processing of 12 samples per BeadChip. The BeadChip is designed to support highly efficient genome-wide association studies and expression-based quantitative trait loci (eQTL) studies.
The high-value content on the HumanHT-12 Expression BeadChips provides genome-wide transcriptional coverage of well-characterized genes, gene candidates, and splice variants, with a significant portion targeting well-established sequences supported by peer-reviewed literature.
Each array on the HumanHT-12 Expression BeadChip targets more than 25,000 annotated genes with more than 48,000 probes derived from the National Center for Biotechnology Information Reference Sequence (NCBI) RefSeq (Build 36.2, Rel 22) and the UniGene (Build 199) databases.
- The twelve-sample format enables large gene expression studies to be completed more quickly and economically
- HumanHT-12 Expression BeadChips use the Direct Hybridization Assay and are scanned on the BeadArray Reader or iScan system.
Applications
Whole-Genome Gene Expression Analysis
The Direct Hybridization Assay offers the highest level of multiplexing for whole-genome expression profiling available. With the most up-to-date expression content and high-throughput processing, Illumina's whole-genome expression arrays produce high-quality data for large gene expression studies, efficiently and economically.
More...Documentation
- Whole-Genome Gene Expression Direct Hybridization Assay Guide
- Whole-Genome Gene Expression Direct Hybridization Assay Experienced User Card
Product Literature
RNA Analysis Solutions: Sample Labeling with TotalPrep from Ambion
The TotalPrep RNA Labeling Kit (available directly from Ambion) has been optimized for use with Illumina's Whole-Genome Expression Solutions. All reagents, including nucleotides, are included, making it even faster and easier to obtain ready-to-hybridize RNA. This streamlined protocol delivers outstanding quality with just a single round of IVT and input requirements of as little as 50 ng total RNA. The TotalPrep RNA Labeling Kit allows you to do more science at a lower cost when partnered with Illumina Gene Expression Solutions.