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HumanRef-8 Expression BeadChip
HumanRef-8 Expression BeadChip
| System: | iScan, BeadArray Reader, HiScanSQ |
|---|---|
| Technology: | BeadArray |
| Assay: | Direct Hybridization Assay |
| Applications: | Whole-Genome Gene Expression Analysis |
| Species: | Human |
| Contents: | Includes 2-6 BeadChips, hybridization and wash buffers, and wash trays |
The HumanRef-8 v3.0 Expression BeadChips enable the generation of genome-wide expression profiles for eight samples on a single BeadChip. The BeadChip targets approximately 24,500 well-annotated transcripts with up-to-date content derived from the National Center for Biotechnology Information Reference Sequence (NCBI RefSeq) database (Build 36.2, Release 22).
The HumanRef-8 Expression BeadChips are arranged in an Array of Arrays format, providing multiple arrays on each slide. The arrays are separated from one another by a seal, so that each array can be hybridized to a different sample. Eight samples can than be interrogated simultaneously
- A minimal amount of total RNA (50-500 ng) is required for the single-round in vitro transcription (IVT) reaction
- HumanRef-8 Expression BeadChips use the Direct Hybridization Assay and are scanned on the Illumina BeadArray Reader or iScan system
- The Whole-Genome DASL Assay is based on the HumanRef-8 Expression BeadChip and enables gene expression analysis of formalin-fixed, paraffin-embedded (FFPE) samples.
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Applications
Whole-Genome Gene Expression Analysis
The Direct Hybridization Assay offers the highest level of multiplexing for whole-genome expression profiling available. With the most up-to-date expression content and high-throughput processing, Illumina's whole-genome expression arrays produce high-quality data for large gene expression studies, efficiently and economically.
The HumanHT-12 v4 Expression BeadChip is Illumina's latest whole-genome expression array, providing coverage for more then 47,000 transcripts and known splice variants across the human transcriptome.
More...Documentation
- Whole-Genome Gene Expression Direct Hybridization Assay Guide
- Whole-Genome Gene Expression Direct Hybridization Assay Experienced User Card
Product Literature
RNA Analysis Solutions: Sample Labeling with TotalPrep from Ambion
The TotalPrep RNA Labeling Kit (available directly from Ambion) has been optimized for use with Illumina's Whole-Genome Expression Solutions. All reagents, including nucleotides, are included, making it even faster and easier to obtain ready-to-hybridize RNA. This streamlined protocol delivers outstanding quality with just a single round of IVT and input requirements of as little as 50 ng total RNA. The TotalPrep RNA Labeling Kit allows you to do more science at a lower cost when partnered with Illumina Gene Expression Solutions.