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Illumina Sequencing Kits v3
Illumina Sequencing Kits v3
| System: | Genome Analyzer, iScanSQ |
|---|---|
| Technology: | Illumina Sequencing |
| Assay: | Single-Read Sequencing, Chromatin Immunoprecipitation Sequencing (ChIP-Seq), mRNA-Seq, Small RNA Discovery and Analysis, Mate-Pair Library Sequencing, Multiplex Sequencing, Paired-End Sequencing |
| Applications: | DNA Sequencing, Gene Regulation Analysis, Sequencing-Based Transcriptome Analysis, SNP Discovery and Structural Variation Analysis, Cytogenetic Analysis, DNA-Protein Interaction Analysis (ChIP-Seq), Sequencing-Based Methylation Analysis, Small RNA Discovery and Analysis |
| Contents: | Kitted reagents for an 18- or 36-cycle run across eight lanes of one flow cell |
Note: Illumina Sequencing v4 Kits are now available
The Genome Analyzer IIx system requires Illumina Sequencing Kits. The improved chemistry of the Sequencing Kits v3 enables faster, longer read length, and high-accuracy sequencing runs across a broad range of applications.
Ready-to-load reagents for use in the Genome Analyzer reduce hands-on preparation time to ten minutes. Sequencing by Synthesis (SBS) technology takes advantage of proprietary, fluorescently-labeled, reversibly terminated nucleotides to sequence the hundreds of millions of clusters, base by base, in parallel.
The 18-Cycle and 36-Cycle Run kits are used for:
- Single-Read Sequencing
- Paired-End Sequencing
- Mate-Pair Library Sequencing
- Multiplex Sequencing
- ChIP-Seq Sequencing
- mRNA-Seq
- Small RNA Discovery and Analysis
Specifications
Learn more about how this product is used.
- mRNA-Seq
- ChIP-Seq Assay
- Mate Pair Sequencing
- Multiplex Sequencing
- Paired-End Sequencing
- Single-Read Sequencing
- Small RNA
Applications
DNA Sequencing
The Genome Analyzer system's powerful combination of read-length and paired-end flexibility enables the broadest range of genomic sequencing applications. Illumina's robust sequencing chemistry supports a wide range of read lengths, allowing researchers to tailor each run to meet their needs.
More...Gene Regulation Analysis
Get a complete view of the epigenome at single-base resolution with the Genome Analyzer. Whether you are interested in CpG methylation, histone modifications, chromatin structure, or DNA-protein interactions, Illumina's unique sequencing chemistry gives you unbiased coverage of every base in the genome.
More...Transcriptome Analysis
Explore the transcriptome like never before. Characterize all transcriptional activity, coding and non-coding, in any organism without a priori assumptions using the Genome Analyzer and achieve an unprecedented view of the transcriptome.
Illumina's unique combination of long and short reads, strand specificity, and millions of counts allows you to annotate coding SNPs, discover transcript isoforms, identify regulatory RNAs, characterize splice junctions, and determine the relative abundance of transcripts.
Generate transcript profiles in a single day with the greatest daily system output. Align RNA sequencing reads across splice junctions and map to reference genomes. Identify and quantify both rare and common transcripts, with six orders of magnitude of dynamic range. Reveal the hidden world of non-coding RNA architecture without prior information.
More...SNP Discovery and Structural Variation Analysis
Single nucleotide polymorphisms (SNPs) and structural variants are at the root of genetic variation among individuals and populations. This variation influences how individuals differ in their risk of disease and their response to therapeutic treatments. SNPs and structural variants are discovered within the genome by comparing multiple genomic sequences from a diverse sample set of individuals. Illumina's industry-leading sequencing technology provides the sample throughput level and high-quality data required for accurate SNP discovery and structural variation analysis studies.
More...Sequencing-Based Methylation Analysis
Identify and track methylation patterns by directly sequencing bisulfite-converted DNA, methylation-sensitive restriction digest-enriched fragments, anti-methyl C-precipitated fragments, or chromatin immunoprecipitates of methyltransferases trapped to aza-labeled DNA on the Genome Analyzer.
More...Small RNA Discovery and Analysis
Small RNA sequencing is a powerful application on the Genome Analyzer, enabling the discovery and profiling of microRNAs and other non-coding RNA on any organism without prior genome annotation. Using low RNA inputs, you can profile the differential expression of known microRNAs as well as detect novel microRNA targets and wide-ranging sequence variation or "iso-miRs" miRBase accessions. With unprecedented sensitivity and dynamic range, Illumina's industry-leading small RNA sequencing methods allow for the most accurate detection and quantification of rare small RNA sequences.
More...Cytogenetic Analysis
Structural variability is a substantial source of genetic variation that has a major influence on phenotypic variation. Cytogenetic analysis allows researchers to profile chromosomal aberrations such as amplifications, deletions, rearrangements, point mutations, copy number changes, and copy-neutral loss of heterozygosity (LOH) events.
More...