Mouse LD Linkage

The mouse has been increasingly recognized as an optimal animal for the study of the genetics of complex disease traits. Two Mouse Linkage Panels (Mouse Low Density [LD] Linkage and Mouse Medium Density [MD] Linkage) are available for mouse genetics applications. These single nucleotide polymorphism (SNP) panels offer a low-cost, efficient, method for attaining genome-wide genetic data to identify quantitative trait loci (QTL), map candidate genes, and increase genotyping throughput.

With the completion of the mouse genome project, greater than 13,000 SNPs have been genotyped across 480 mouse strains using the Illumina GoldenGate Genotyping Assay. This SNP set is available from the Wellcome-CTC Mouse Strain SNP Genotype Set.

The Mouse LD Linkage Panel consists of 377 loci and has been optimized for application to N2 and F2 mouse genetics crosses, those typically used for mapping QTLs. This panel provides approximately 175-200 informative markers per cross and covers the entire mouse genome.

The Mouse LD Linkage Panel was designed to include approximately four SNPs per 27 Mb interval. At least one SNP in each interval was chosen to be informative in crosses involving the C57Bl/6J strain. On average, at least one SNP in each interval is informative for 85% of all possible strain combinations.

• The Mouse LD Linkage Panel can be used on 12-, 16-, and 32-sample Universal BeadChips or the 96-sample Universal Array Matrix
• SNP sequences are optimized for Illumina's proven GoldenGate Genotyping Assay protocol