Illumina MouseRef-8 v2.0 Expression BeadChips

MouseRef-8 v2.0 Expression BeadChip Kits

System: iScan, BeadArray Reader, HiScanSQ
Technology: BeadArray
Assay: Direct Hybridization Assay
Applications: Whole-Genome Gene Expression Analysis
Species: Mouse
Contents: Includes BeadChips, hybridization and wash buffers, and wash trays

Illumina's MouseRef-8 v2.0 Expression BeadChip offers the most up-to-date content for whole-genome expression profiling in the mouse. This BeadChip targets approximately 25,600 well-annotated RefSeq transcripts, over 19,100 unique genes, and enables the interrogation of eight samples in parallel.

The MouseRef-8 BeadChip content is derived from the National Center for Biotechnology Information Reference Sequence (NCBI RefSeq) database (Build 36, Release 22), supplemented with probes derived from the Mouse Exonic Evidence Based Oligonucliotide (MEEBO) set as well as exemplar protein-coding sequences described in the RIKEN FANTOM2 database.

 

Order Information

BD-202-0202
Mouse
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Required Products

Specifications

Learn more about how this product is used.


Contents of Whole-Genome Gene Expression Kits:
Catalog Number
Whole-Genome Expression Kits (2)
6-, 8-, and 12- sample formats
Mouse BD-201-0202, BD-202-0202
WHole-Genome Expression Kits (6)
6-, 8-, and 12- sample formats
Mouse BD-201-0602, BD-202-0602

Ship at RT. Store at RT.
E1BC (Wash E1 BC Buffer)
20 ml (4 x 5 ml)
20 ml (4 x 5 ml)
BeadChip Tweezer
1
1
Wash Trays
14
14
Wash Tray Lids
7
7
HTW (High-Temperature Wash Buffer)
325 ml
325 ml
Ship at RT. Store at 2° to 8°C.
Block E1 (Blocking E1 Buffer)
40 ml
40 ml
Whole-Genome Expression BeadChips
2
6
Ship on dry ice. Store at -15° to -25°C.
HYB (Hybridization E1 Buffer)
1.7 ml
1.7 ml
HCB (Humidity Control Buffer)
2.8 ml
2.8 ml

Probe Content Source:
  BeadChip
MouseWG-6
MouseRef-8
  
RefSeq
34,526
24,477
RIKEN
4,922
769
Meebo
5,833
1,220
Total
45,281
25,697
 

Applications

RNA Analysis Solutions: Sample Labeling with TargetAmp-Nano from Epicentre (an Illumina company)

The TargetAmp-Nano Labeling Kit for Illumina Expression BeadChips (available from Epicentre) produces micrograms of biotin-aRNA from as little as 25 ng of total RNA. The easy six-hour reaction enables researchers to prepare biotin-aRNA and begin BeadChip hybridizations in the same day. Biotin-aRNA produced by the TargetAmp-Nano Kit provides sensitive gene detection and yields consistent differential expression results.



RNA Analysis Solutions: Sample Labeling with TotalPrep from Ambion

The TotalPrep RNA Labeling Kit (available directly from Ambion) has been optimized for use with Illumina's Whole-Genome Expression Solutions. All reagents, including nucleotides, are included, making it even faster and easier to obtain ready-to-hybridize RNA. This streamlined protocol delivers outstanding quality with just a single round of IVT and input requirements of as little as 50 ng total RNA. The TotalPrep RNA Labeling Kit allows you to do more science at a lower cost when partnered with Illumina Gene Expression Solutions.