Products /
Multiplexing Sample Preparation Oligonucleotide Kit
Multiplexing Sample Preparation Oligonucleotide Kit
| System: | Genome Analyzer, iScanSQ |
|---|---|
| Technology: | Illumina Sequencing |
| Assay: | Multiplex Sequencing |
| Applications: | DNA Sequencing, Gene Regulation Analysis, SNP Discovery and Structural Variation Analysis, Cytogenetic Analysis, Sequencing-Based Methylation Analysis |
| Contents: | Oligonucleotides for tagging up to 96 prepared samples |
The Multiplexing Sample Preparation Oligonucleotide Kit contains 12 unique oligonucleotides to "tag" libraries for pooling in a single lane of a flow cell. Using this kit, up to 96 samples can be sequenced on a single flow cell using the Genome Analyzer. Each oligo is six bases in length, sufficient for accurate differentiation between tags and to overcome any single-base errors that may inadvertently be introduced during PCR.
- Single-read and paired-end read libraries can be produced
- The Multiplexing Sample Preparation Oligonucleotide Kit requires the Multiplexing Sequencing Primers and PhiX Control Kit, a Genomic DNA or Paired-End Read Sample Prep Kit, Standard Cluster Generation Kit or Paired-End Cluster Generation Kit, and 18- and 36-Cycle Illumina Sequencing Kits
Applications
Sequencing
Illumina sequencing gives you the power to comprehensively characterize the genome, epigenome or the transcriptome. Go from DNA to data in under a week with less than four hours of hands-on time. Applications flexibility for any genetic variant. Superior raw-read accuracy and the industry’s simplest, automated workflow. It’s sequencing power for every researcher.
More...Gene Regulation Analysis
Get a complete view of the epigenome at single-base resolution with the Genome Analyzer. Whether you are interested in CpG methylation, histone modifications, chromatin structure, or DNA-protein interactions, Illumina's unique sequencing chemistry gives you unbiased coverage of every base in the genome.
More...SNP Discovery and Structural Variation Analysis
Single nucleotide polymorphisms (SNPs) and structural variants are at the root of genetic variation among individuals and populations. This variation influences how individuals differ in their risk of disease and their response to therapeutic treatments. SNPs and structural variants are discovered within the genome by comparing multiple genomic sequences from a diverse sample set of individuals. Illumina's industry-leading sequencing technology provides the sample throughput level and high-quality data required for accurate SNP discovery and structural variation analysis studies.
More...Cytogenetic Analysis
Structural variability is a substantial source of genetic variation that has a major influence on phenotypic variation. Cytogenetic analysis allows researchers to profile chromosomal aberrations such as amplifications, deletions, rearrangements, point mutations, copy number changes, and copy-neutral loss of heterozygosity (LOH) events.
More...Array-Based Methylation Analysis
DNA methylation plays a critical role in the regulation of gene expression and has been implicated in the etiology of many human diseases including cancer. Methylation profiling with BeadArray technology allows researchers to analyze the effects of aberrant methylation (either hyper- or hypomethylation) for a variety of applications. Illumina has developed a robust methylation profiling platform that provides quantitative methylation measurement at the single-CpG-site level, providing the highest resolution for understanding epigenetic changes.
More...Sequencing-Based Methylation Analysis
Identify and track methylation patterns by directly sequencing bisulfite-converted DNA, methylation-sensitive restriction digest-enriched fragments, anti-methyl C-precipitated fragments, or chromatin immunoprecipitates of methyltransferases trapped to aza-labeled DNA on the Genome Analyzer.
More...