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Multiplexing Sequencing Primers and PhiX Control Kit V2
| System: | HiSeq 2000, HiScanSQ, Genome Analyzer IIx, HiSeq 1000 |
|---|---|
| Technology: | Illumina Sequencing |
| Assay: | Multiplex Sequencing |
| Applications: | DNA Sequencing, Gene Regulation Analysis, SNP Discovery and Structural Variation Analysis, Cytogenetic Analysis, Sequencing-Based Methylation Analysis |
| Contents: | Multiplexing sequencing primers, multiplexing control DNA, and buffer set |
The Multiplexing Sequencing Primers and PhiX Control Kit V2 is used to sequence samples tagged by using the Multiplexing Sample Preparation Oligonucleotide Kit and then amplified in the cBot or Cluster Station. Sequencing is fully automated on an Illumina sequencing system.
Order Information
Specifications
Learn more about how this product is used.
| Contents of the multiplexing Sequencing primers and phix control kit: | |
|---|---|
| Catalog Number |
PE-400-1002
|
| Ship on dry ice. Store at -15° to -25°C. | |
| Index Sequencing Primer |
120 µl (100 µM)
|
| Multiplexing Rd2 Sequencing Primer |
120 µl (100 µM)
|
| Multiplexing PhiX Control |
15 µl (10 nM)
|
| Ship at RT. Store at 2° to 8°C. | |
| NaOH |
30 ml (0.1 N)
|
| TE Buffer |
20 ml
|
| Wash Buffer |
37 ml
|
| Hybridization Buffer |
12 ml
|
Applications
Gene Regulation Analysis
Get a complete view of the epigenome at single-base resolution with the Genome AnalyzerIIx. Whether you are interested in CpG methylation, histone modifications, chromatin structure, or DNA-protein interactions, Illumina's unique sequencing chemistry gives you unbiased coverage of every base in the genome.
More...SNP Discovery and Structural Variation Analysis
Single nucleotide polymorphisms (SNPs) and structural variants are at the root of genetic variation among individuals and populations. This variation influences how individuals differ in their risk of disease and their response to therapeutic treatments. SNPs and structural variants are discovered within the genome by comparing multiple genomic sequences from a diverse sample set of individuals. Illumina's industry-leading sequencing technology provides the sample throughput level and high-quality data required for accurate SNP discovery and structural variation analysis studies.
More...Sequencing-Based Methylation Analysis
Identify and track methylation patterns by directly sequencing bisulfite-converted DNA, methylation-sensitive restriction digest-enriched fragments, anti-methyl C-precipitated fragments, or chromatin immunoprecipitates of methyltransferases trapped to aza-labeled DNA on the Illumina's portfolio of sequencing systems.
More...Cytogenetic Analysis
Structural variability is a substantial source of genetic variation that has a major influence on phenotypic variation. Cytogenetic analysis allows researchers to profile chromosomal aberrations such as amplifications, deletions, rearrangements, point mutations, copy number changes, and copy-neutral loss of heterozygosity (LOH) events.
More...Array-Based Methylation Analysis
DNA methylation plays a critical role in the regulation of gene expression and has been implicated in the etiology of many human diseases including cancer. Methylation profiling with BeadArray technology allows researchers to analyze the effects of aberrant methylation (either hyper- or hypomethylation) for a variety of applications. Illumina has developed a robust methylation profiling platform that provides quantitative methylation measurement at the single-CpG-site level, providing the highest resolution for understanding epigenetic changes.
More...
At Illumina, our goal is to apply innovative technologies and revolutionary assays to the analysis of genetic variation and function, making studies possible that were not even imaginable just a few years ago. These studies will help make the realization of personalized medicine possible. With such rapid advances in technology taking place, it is mission critical to have solutions that are not only innovative, but flexible, scalable, and complete with industry-leading support and service. As a global company that places high value on collaborative interactions, rapid delivery of solutions, and prioritizing the needs of its customers, we strive to meet this challenge. Illumina's innovative, array-based solutions for DNA, RNA, and protein analysis serve as tools for disease research, drug development, and the development of molecular tests in the clinic.