Illumina Multiplexing Sequencing Primers and PhiX Control Kit

Multiplexing Sequencing Primers and PhiX Control Kit V2

System: Genome Analyzer, HiScanSQ
Technology: Illumina Sequencing
Assay: Multiplex Sequencing
Applications: DNA Sequencing, Gene Regulation Analysis, SNP Discovery and Structural Variation Analysis, Cytogenetic Analysis, Sequencing-Based Methylation Analysis
Contents: Multiplexing sequencing primers, multiplexing control DNA, and buffer set

The Multiplexing Sequencing Primers and PhiX Control Kit V2 is used to sequence samples tagged using the Multiplexing Sample Preparation Oligonucleotide Kit.

  • Using this kit, up to 10 sequencing runs can be performed on the Genome Analyzer

Order Information

PE-400-2002
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Required Products

Specifications

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Contents of the Multiplexing Sample Preparation Oligonucleotide Kit, contents of the Multiplex Sequencing Primers and PhiX Control Kit, required Reagents for Multiplex Sequencing Applications

Applications

Gene Regulation Analysis

Get a complete view of the epigenome at single-base resolution with the Genome Analyzer. Whether you are interested in CpG methylation, histone modifications, chromatin structure, or DNA-protein interactions, Illumina's unique sequencing chemistry gives you unbiased coverage of every base in the genome.

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SNP Discovery and Structural Variation Analysis

Single nucleotide polymorphisms (SNPs) and structural variants are at the root of genetic variation among individuals and populations. This variation influences how individuals differ in their risk of disease and their response to therapeutic treatments. SNPs and structural variants are discovered within the genome by comparing multiple genomic sequences from a diverse sample set of individuals. Illumina's industry-leading sequencing technology provides the sample throughput level and high-quality data required for accurate SNP discovery and structural variation analysis studies.

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Sequencing-Based Methylation Analysis

Identify and track methylation patterns by directly sequencing bisulfite-converted DNA, methylation-sensitive restriction digest-enriched fragments, anti-methyl C-precipitated fragments, or chromatin immunoprecipitates of methyltransferases trapped to aza-labeled DNA on the Genome Analyzer.

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Cytogenetic Analysis

Structural variability is a substantial source of genetic varia­tion that has a major influence on phenotypic variation. Cytogenetic analysis allows researchers to profile chromosomal aberrations such as amplifications, deletions, rearrangements, point mutations, copy number changes, and copy-neutral loss of heterozygosity (LOH) events.

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Array-Based Methylation Analysis

DNA methylation plays a critical role in the regulation of gene expression and has been implicated in the etiology of many human diseases including cancer. Methylation profiling with BeadArray technology allows researchers to analyze the effects of aberrant methylation (either hyper- or hypomethylation) for a variety of applications. Illumina has developed a robust methylation profiling platform that provides quantitative methylation measurement at the single-CpG-site level, providing the highest resolution for understanding epigenetic changes.

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