Nextera DNA Sample Preparation Kits provide the fastest and easiest workflow, enabling sequencing-ready libraries to be generated in less than 90 minutes, with less than 15 minutes of hands-on time. DNA is simultaneously fragmented and tagged with sequencing adapters in a single step, using standard lab equipment. Libraries prepared with Nextera kits are compatible with all Illumina sequencers.

The industry's fastest sample prep.

MiSeq: Prep 1.5 hours, Sequence 4.5 hours, Analyze less than 2 hours

View the workflow »
Workflow shown using the Nextera Sample Preparation Kit, and 1 x 36 bp sequencing on MiSeq system.

With Nextera technology, DNA is simultaneously fragmented and tagged with sequencing adapters in a single step, using standard lab equipment. Ideal for precious samples available in limited quantity, the protocol requires only 50 ng of DNA input. Libraries prepared with Nextera kits are compatible with all Illumina sequencers.

The TruSeq Dual Index Sequencing Primer Box is required for single-read and paired-end sequencing of Nextera libraries on HiSeq 1000/2000, HiScanSQ, and Genome Analyzer IIx instruments. These primers are required for non-indexed, single-indexed, and dual-indexed Nextera libraries.

Please note that a Nextera Index kit (FC-121-1011 or FC-121-1012) is required to complete the protocol, regardless of the sample pooling level used for sequencing.

System Compatibility

Genome Analyzer IIx
HiScanSQ
HiSeq 1000
HiSeq 2000
HiSeq 2500
MiSeq Desktop Sequencer
NextSeq 500 Sequencing System

Catalog IDs: FC-121-1031, FC-121-1030, FC-121-1012, FC-121-1011, FC-121-1003, PE-121-1003

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