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Paired-End Cluster Generation Kit V4
Paired-End Cluster Generation Kit V4
| System: | Genome Analyzer, HiScanSQ |
|---|---|
| Technology: | Illumina Sequencing |
| Assay: | Paired-End Sequencing |
| Applications: | DNA Sequencing, Gene Regulation Analysis, SNP Discovery and Structural Variation Analysis, Cytogenetic Analysis, Sequencing-Based Methylation Analysis |
| Contents: | Reagents and consumables for processing 1-10 runs on the original Genome Analyzer system or the Genome Analyzer II system |
The Paired-End Cluster Generation Kit v4 provides reagents for cluster generation on paired-end flow cells with cBot of the Cluster Station. The kit also includes reagents for resynthesis of the reverse strand on the Genome Analyzer. The master-mixed reagents in this kit provide a streamlined workflow with a total of 20 minutes hands-on time for reagent preparation and setup.
DNA library samples are bound to complementary adapter oligos grafted on the surface of the Illumina Genome Analyzer flow cell. The templates are copied from the hybridized primer by 3’ extension using a high fidelity DNA polymerase to prevent misincorporation errors. These are isothermally amplified to create clonal clusters of ~1,000 copies each, ready for sequencing on the Genome Analyzer.
The paired-end flow cell contains proprietary oligos that enable selective cleavage of the forward DNA strand after resynthesis of the reverse strand. The reverse strand is regenerated by bridge amplification with the Paired-End Module (attached to the Genome Analyzer). After resynthesis of the reverse strand, the original forward strand is cleaved and the reverse strand is sequenced for the second read.
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Sequencing
Illumina sequencing gives you the power to comprehensively characterize the genome, epigenome or the transcriptome. Go from DNA to data in under a week with less than four hours of hands-on time. Applications flexibility for any genetic variant. Superior raw-read accuracy and the industry’s simplest, automated workflow. It’s sequencing power for every researcher.
More...Gene Regulation Analysis
Get a complete view of the epigenome at single-base resolution with the Genome Analyzer. Whether you are interested in CpG methylation, histone modifications, chromatin structure, or DNA-protein interactions, Illumina's unique sequencing chemistry gives you unbiased coverage of every base in the genome.
More...SNP Discovery and Structural Variation Analysis
Single nucleotide polymorphisms (SNPs) and structural variants are at the root of genetic variation among individuals and populations. This variation influences how individuals differ in their risk of disease and their response to therapeutic treatments. SNPs and structural variants are discovered within the genome by comparing multiple genomic sequences from a diverse sample set of individuals. Illumina's industry-leading sequencing technology provides the sample throughput level and high-quality data required for accurate SNP discovery and structural variation analysis studies.
More...Sequencing-Based Methylation Analysis
Identify and track methylation patterns by directly sequencing bisulfite-converted DNA, methylation-sensitive restriction digest-enriched fragments, anti-methyl C-precipitated fragments, or chromatin immunoprecipitates of methyltransferases trapped to aza-labeled DNA on the Genome Analyzer.
More...Cytogenetic Analysis
Structural variability is a substantial source of genetic variation that has a major influence on phenotypic variation. Cytogenetic analysis allows researchers to profile chromosomal aberrations such as amplifications, deletions, rearrangements, point mutations, copy number changes, and copy-neutral loss of heterozygosity (LOH) events.
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