- MyIllumina
- Tools
The Paired-End DNA Sample Preparation Kit provides reagents to prepare DNA libraries with insert sizes from 200-500 bp for paired-end sequencing. The kit provides reagents for repairing the ends of DNA that have been fragmented by nebulization or sonication.
Ends are repaired with a combination of fill-in reactions and exonuclease activity to produce blunt ends. An 'A'- base is added to the blunt ends followed by ligation to Illumina Paired-End Sequencing adapters. These adapters contain two unique sequencing primer hybridization sites.
Additional sequences complementary to the oligonucleotides in the flow cell are added to the adapter sequences with tailed PCR primers, followed by gel-based size selection and purification. These libraries are ready to load on cBot or the Cluster Station for cluster generation.
Catalog IDs: PE-102-1001, PE-102-1002
View the product support page for additional technical information ยป
At Illumina, our goal is to apply innovative technologies and revolutionary assays to the analysis of genetic variation and function, making studies possible that were not even imaginable just a few years ago. These studies will help make the realization of personalized medicine possible. With such rapid advances in technology taking place, it is mission critical to have solutions that are not only innovative, but flexible, scalable, and complete with industry-leading support and service. As a global company that places high value on collaborative interactions, rapid delivery of solutions, and prioritizing the needs of its customers, we strive to meet this challenge. Illumina's innovative, array-based solutions for DNA, RNA, and protein analysis serve as tools for disease research, drug development, and the development of molecular tests in the clinic.