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Paired-End DNA Sample Prep Kit V1
Paired-End DNA Sample Prep Kit V1
| System: | Genome Analyzer, HiScanSQ |
|---|---|
| Technology: | Illumina Sequencing |
| Assay: | Paired-End Sequencing |
| Applications: | DNA Sequencing, Gene Regulation Analysis, Cytogenetic Analysis, Sequencing-Based Methylation Analysis |
| Contents: | Reagents for library preparation of 10-40 DNA samples |
The Paired-End DNA Sample Prep Kit v1 is used to build DNA libraries with insert sizes from 200-500 bp for paired-end sequencing. The kit provides reagents for repairing the ends of DNA that have been fragmented by nebulization or sonication.
Ends are repaired with a combination of fill-in reactions and exonuclease activity to produce blunt ends. An 'A'- base is added to the blunt ends followed by ligation to Illumina Paired-End Sequencing adapters. These adapters contain two unique sequencing primer hybridization sites.
Additional sequences complementary to the oligonucleotides in the flow cell are added to the adapter sequences with tailed PCR primers. This is followed by gel-based size selection and purification to create libraries for cluster generation on cBot or the Cluster Station.
- Use the Paired-End DNA Sample Prep Kit with a Paired-End Cluster Generation Kit and the appropriate combination of 18-cycle and 36-cycle Illumina Sequencing Kits to achieve the target number of cycles
Order Information
Applications
Sequencing
Illumina sequencing gives you the power to comprehensively characterize the genome, epigenome or the transcriptome. Go from DNA to data in under a week with less than four hours of hands-on time. Applications flexibility for any genetic variant. Superior raw-read accuracy and the industry’s simplest, automated workflow. It’s sequencing power for every researcher.
More...Gene Regulation Analysis
Get a complete view of the epigenome at single-base resolution with the Genome Analyzer. Whether you are interested in CpG methylation, histone modifications, chromatin structure, or DNA-protein interactions, Illumina's unique sequencing chemistry gives you unbiased coverage of every base in the genome.
More...Cytogenetic Analysis
Structural variability is a substantial source of genetic variation that has a major influence on phenotypic variation. Cytogenetic analysis allows researchers to profile chromosomal aberrations such as amplifications, deletions, rearrangements, point mutations, copy number changes, and copy-neutral loss of heterozygosity (LOH) events.
More...Sequencing-Based Methylation Analysis
Identify and track methylation patterns by directly sequencing bisulfite-converted DNA, methylation-sensitive restriction digest-enriched fragments, anti-methyl C-precipitated fragments, or chromatin immunoprecipitates of methyltransferases trapped to aza-labeled DNA on the Genome Analyzer.
More...Array-Based Methylation Analysis
DNA methylation plays a critical role in the regulation of gene expression and has been implicated in the etiology of many human diseases including cancer. Methylation profiling with BeadArray technology allows researchers to analyze the effects of aberrant methylation (either hyper- or hypomethylation) for a variety of applications. Illumina has developed a robust methylation profiling platform that provides quantitative methylation measurement at the single-CpG-site level, providing the highest resolution for understanding epigenetic changes.
More...Documentation
- Paired-End Sequencing Sample Preparation Guide (English)
- Paired-End Sequencing Sample Preparation Guide (Japanese)
Product Literature
- HiSeq 2000 Specification Sheet
- Genome Analyzer IIx Data Sheet
- Genome Analyer IIe Specification Sheet
- HiScanSQ Specification Sheet
- Sequencing Reagent Shelf Life Reference Tool
- Calling Sequencing SNPs
- GenomeStudio Software DNA Sequencing Module Workflow
- iScan System and AutoLoader 2.x Data Sheet
- Molecular Cytogenetics