| System: | iScan, iScanSQ, BeadStation, BeadArray Reader |
|---|---|
| Technology: | BeadArray |
| Assay: | Infinium DNA Analysis Assay |
| Applications: | Focused Genotyping |
| Species: | Porcine |
| Contents: | Each package contains 4 to 96 BeadChips, along with reagents for amplifying, fragmenting, hybridizing, labeling, and detecting 48 to 1152 samples |
The PorcineSNP60 BeadChip features 62,163 evenly spaced probes offering comprehensive coverage of the porcine genome. The BeadChip was developed through Illumina's iSelect program in collaboration with leading porcine researchers from both US and International Universities and Research Institutes including Iowa State University, the University of Illinois, Cambridge University, and Wageningen University.
The Porcine SNP Chip Consortium made the final selection of the highest quality markers from more than 510,000 single nucleotide polymorphisms (SNPs), after combining novel discovered SNPs with several other existing databases and study results.
The panel was optimized using multiple criteria for marker selection, including minor allele frequency determined from representative sample sequencing, allele count, quality score, spacing, location, and validation status. More than 43,000 SNPs on the final panel were discovered using deep sequencing with the Illumina Genome Analyzer.
Illumina's Infinium High-Density DNA Analysis Solution combines the Infinium Assay with BeadChips to allow large-scale interrogation of genome variations, accelerating discovery of disease-related genetic regions. The Infinium Assay interrogates a large number of single nucleotide polymorphisms (SNPs) at unlimited levels of loci multiplexing.
Focused genotyping supports a variety of applications such as candidate-gene studies in cancer, cardiovascular disease, and admixture mapping. Illumina also works closely with major animal consortia to develop genome-wide genotyping products for non-human organisms, including both animal and plant species.
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