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Small RNA Cluster Generation Kits
Small RNA Cluster Generation Kits
| System: | Genome Analyzer, HiScanSQ |
|---|---|
| Technology: | Illumina Sequencing |
| Assay: | Small RNA Discovery and Analysis |
| Applications: | Sequencing-Based Transcriptome Analysis, Small RNA Discovery and Analysis |
| Species: | Any |
| Contents: | Contains reagents and consumables for processing flow cells of Small RNA libraries in the Genome Analyzer system |
With Small RNA Discovery and Analysis, Illumina scientists have designed a sample preparation protocol that enables sequencing of known and novel microRNAs as well as other non-coding RNAs, such as piwi-interacting RNAs and siRNAs.
The Small RNA Cluster Generation Kit provides reagents for cluster amplification on single-read flow cells. The small RNA library samples are bound to complementary adapter oligonucleotides grafted on the surface of the Illumina Genome Analyzer flow cell. These are isothermally amplified to create clonal clusters of ~1,000 copies each, ready for sequencing on the Genome Analyzer.
A Small RNA Library-specific sequencing primer is included for use with small RNA libraries. The master-mixed reagents in this kit provide a streamlined workflow with a total of 10 minutes hands-on time for reagent preparation and setup.
- Once reagents are loaded, the fully automated cluster generation process takes <4 hours
- Use with the Small RNA Sample Prep Kit and 36-Cycle Illumina Sequencing Kit
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Applications
Transcriptome Analysis
Explore the transcriptome like never before. Characterize all transcriptional activity, coding and non-coding, in any organism without a priori assumptions using the Genome Analyzer and achieve an unprecedented view of the transcriptome.
Illumina's unique combination of long and short reads, strand specificity, and millions of counts allows you to annotate coding SNPs, discover transcript isoforms, identify regulatory RNAs, characterize splice junctions, and determine the relative abundance of transcripts.
Generate transcript profiles in a single day with the greatest daily system output. Align RNA sequencing reads across splice junctions and map to reference genomes. Identify and quantify both rare and common transcripts, with six orders of magnitude of dynamic range. Reveal the hidden world of non-coding RNA architecture without prior information.
More...Small RNA Discovery and Analysis
Small RNA sequencing is a powerful application on the Genome Analyzer, enabling the discovery and profiling of microRNAs and other non-coding RNA on any organism without prior genome annotation. Using low RNA inputs, you can profile the differential expression of known microRNAs as well as detect novel microRNA targets and wide-ranging sequence variation or "iso-miRs" miRBase accessions. With unprecedented sensitivity and dynamic range, Illumina's industry-leading small RNA sequencing methods allow for the most accurate detection and quantification of rare small RNA sequences.
More...Transcriptome Analysis
Explore the transcriptome like never before. Characterize all transcriptional activity, coding and non-coding, in any organism without a priori assumptions using the Genome Analyzer and achieve an unprecedented view of the transcriptome. Illumina's unique combination of long and short reads, strand specificity, and millions of counts allows you to annotate coding SNPs, discover transcript isoforms, identify regulatory RNAs, characterize splice junctions, and determine the relative abundance of transcripts. Generate transcript profiles in a single day with the greatest daily system output. Align RNA sequencing reads across splice junctions and map to reference genomes. Identify and quantify both rare and common transcripts, with six orders of magnitude of dynamic range. Reveal the hidden world of non-coding RNA architecture without prior information.
More...