TruSeq SBS kit-HS

TruSeq SBS kit-HS

System: HiSeq 2000, HiScanSQ, cBot, HiSeq 1000
Technology: Illumina Sequencing
Assay: Single-Read Sequencing, mRNA-Seq, Mate-Pair Library Sequencing, Multiplex Sequencing, Paired-End Sequencing
Applications: DNA Sequencing, Gene Regulation Analysis, SNP Discovery and Structural Variation Analysis, Cytogenetic Analysis, Sequencing-Based Methylation Analysis
Contents: Two kit sizes are available for either 58 or 209 cycles of sequencing on the HiSeq 2000 and HiScanSQ sequencing systems

The HiSeq 2000, HiSeq 1000, and HiScanSQ sequencing systems require the TruSeq SBS Kit-HS . Pre-configured reagents reduce hands-on preparation time to two minutes. Sequencing by synthesis (SBS) technology takes advantage of proprietary, fluorescently-labeled, reversibly terminated nucleotides to sequence the hundreds of millions of clusters, base by base, in parallel.

Note: The TruSeq SBS Kit-HS is NOT compatible with the Genome Analyzer.


Order Information

FC-401-1001
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Required Products

Specifications

Contents of the TruSeq SBS Kit-HS
Catalog Number FC-401-1001
Box 1
Ship on dry ice.
Store at -15° to -20°C.
CMR (Cleavage Mix Reagent)
SMR (Scanning Mix Reagent)
LRP (Long Read DNA Polymerase)
LFN36 (Long Read Nucleotide Mix)
Box 2
Ship on dry ice.
Store at 2° to 8°C.
PW1 (Wash Solution)
ICR (Incorporation Mix Reagent)
SB1 (High Salt Buffer)
SB2 (Incorporation Buffer)
SB3 (Cleavage Buffer)


Applications

DNA Sequencing

Illumina’s sequencers powerfully combine the flexibility of single reads, short- and long-insert paired-end reads, enabling the broadest range of genomic applications. TruSeq sequencing chemistry supports this wide range of applications including whole-genome sequencing, targeted resequencing, de novo sequencing, SNP discovery, identification of copy number variations, and chromosomal rearrangement.

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Gene Regulation Analysis

Get a complete view of the epigenome at single-base resolution with the Genome AnalyzerIIx. Whether you are interested in CpG methylation, histone modifications, chromatin structure, or DNA-protein interactions, Illumina's unique sequencing chemistry gives you unbiased coverage of every base in the genome.

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SNP Discovery and Structural Variation Analysis

Single nucleotide polymorphisms (SNPs) and structural variants are at the root of genetic variation among individuals and populations. This variation influences how individuals differ in their risk of disease and their response to therapeutic treatments. SNPs and structural variants are discovered within the genome by comparing multiple genomic sequences from a diverse sample set of individuals. Illumina's industry-leading sequencing technology provides the sample throughput level and high-quality data required for accurate SNP discovery and structural variation analysis studies.

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Sequencing-Based Methylation Analysis

Identify and track methylation patterns by directly sequencing bisulfite-converted DNA, methylation-sensitive restriction digest-enriched fragments, anti-methyl C-precipitated fragments, or chromatin immunoprecipitates of methyltransferases trapped to aza-labeled DNA on the Illumina's portfolio of sequencing systems.

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Cytogenetic Analysis

Structural variability is a substantial source of genetic varia­tion that has a major influence on phenotypic variation. Cytogenetic analysis allows researchers to profile chromosomal aberrations such as amplifications, deletions, rearrangements, point mutations, copy number changes, and copy-neutral loss of heterozygosity (LOH) events.

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