Whole-Genome DASL Assay Kits
| System: | iScan, BeadArray Reader, HiScanSQ |
|---|---|
| Technology: | BeadArray |
| Assay: | Whole-Genome DASL Assay |
| Applications: | FFPE Sample Analysis |
| Species: | Human |
| Contents: | Includes DAP, HumanRef-8 BeadChips, and reagents for amplifying, hybridizing, washing, and processing RNA samples. Available with or without UDG enzyme. |
The Whole-Genome DASL Assay leverages the unique PCR and labeling steps of the DASL Assay for sensitive, reproducible, cost-effective gene expression profiling of low-abundance and partially degraded RNA, such as that found in formalin-fixed paraffin-embedded (FFPE) samples. Over 24,000 targets can be analyzed at once.
The Whole-Genome DASL Assay begins with the conversion of total RNA to cDNA using biotinylated oligo(dT) and random nonamer primers. The biotinylated cDNA is annealed to the DASL Assay Pool (DAP) probe groups that contain oligonucleotides specifically designed to interrogate each target sequence in the transcripts. These probes span about 50 bases, making it possible to profile partially degraded RNA.
Following this, correctly annealed, assay-specific, oligos are extended and ligated to generate amplifiable products. These templates are labeled during PCR amplification by including fluorescent primers in the reaction. The resulting PCR products are scanned using the BeadArray Reader or iScan System to determine the presence or absence of specific genes.
- The amount of RNA recommended for the DASL Assay can vary from 100 ng per assay for intact RNA to 200 ng of total RNA for partially degraded RNA.
Specifications
Learn more about how this product is used.
| Contents of Whole-genome DASL Assay Kit: | |
|---|---|
| Catalog Number |
DA-903-0024
DA-903-1024 (W/UDG) DA-903-0096
DA-903-1096 (W/UDG) |
| Number of Samples |
24
96
|
| Ship at -65° to -85°C. Store at -15° to -25°C. | |
| MCS3 (cDNA Synthesis Master Mix 3) |
320 µl
680 µl
|
| OB1 (Oligo Hybridization and DNA Binding Buffer 1) |
3.5 ml
3.5 ml
|
| AM1 (Add Master Mix for Extension/Ligation 1 Reagent) |
4.0 ml
4.0 ml
|
| IP1 (Innoculate PCR Reagent) |
3.8 ml
3.8 ml
|
| UDG (Uracil DNA Glycosylase*) |
52 µl
52 µl
|
| SCM (Single Color Master Mix Reagent) |
3.2 ml
3.2 ml
|
| XC4 (XStain BeadChip Solution 4) |
350 ml
350 ml
|
| HCB (Humidity Control Buffer) |
2.8 ml
5.6 ml (2 x 2.8 ml)
|
| HYB (Hybridization Buffer) |
1.7 ml
1.7 ml
|
| Ship at -65° to -85°C. Store at -15° to -25°C. | |
| AM1 (Add Master Mix for Extension/Ligation 1 Reagent) |
15 ml
15 ml
|
| UB1 (Universal Buffer 1) |
25 ml
25 ml
|
| PS1 (Precipitation Solution 1) |
30 ml
30 ml
|
| MPB (Paramagnetic Particles B) |
2.2 ml
72.2 ml
|
| HumanRef-8 BeadChips |
3
12
|
| Ship at -65° to -85°C. Store at 2° to 8°C. | |
| MH1 (Make Hyb1 Reagent) |
7.0 ml (2 x 3.5 ml)
7.0 ml (24 x 3.5 ml)
|
| WC1 (Wash BeadChip Buffer) |
20.0 ml (4 x 5.0 ml)
20.0 ml (4 x 5.0 ml)
|
| HTW (High-Temperature Wash Buffer) |
325 ml
325 ml
|
| UB2 (Universal Buffer 2) |
300 ml
300 ml
|
| PB1 (Preparing BeadChip for Hybridization Buffer) |
950 ml
950 ml
|
|
*Supplied in DA-903-1024 and DA-903-1096 only. |
|
Applications
FFPE Sample Analysis
Formalin-fixed, paraffin-embedded (FFPE) samples are preserved tissue samples that are generally associated with disease. Many of these samples represent clinical outcomes, which could provide a potential gold mine of information when linked with underlying expression profiles. FFPE samples generally contain partially degraded RNA, so transcription analysis is a challenge for many gene expression assays. By using unique PCR and labeling steps based on the proven GoldenGate chemistry, Illumina's DASL assays provide high-quality data from degraded RNA samples. The DASL assay is available in the Whole-Genome DASL Assay, a fixed content, whole-genome profiling panel.
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