Sue Forrest, Ph.D.
"I think next-generation sequencing has just started to make people think about how to do science differently"

Sue Forrest, Ph.D.
Chief Executive Officer, Australian Genome Research Facility

Dr. Sue Forrest is the Chief Executive Officer of the Australian Genome Research Facility (AGRF). The institution provides a range of genomic services and is involved in a number of interesting projects throughout Australia.


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Q&A with Dr. Sue Forrest

Please tell us about AGRF

AGRF is the largest service provider for genomics in Australia. AGRF is a not for profit organization and is supported by federal and state government grants as well as revenue from doing project work. We've been in existence for over 12 years now, so we've lived through many changes in genome science. We originally started out as a Sanger sequencing and microsatellite genotyping service provider in two of our State nodes. Since then we've really escalated in terms of the different services that we offer, while branching out to other major cities.

Are there any recent projects that you found particularly interesting?

At AGRF we believe that with these new technologies it's important to showcase what they can do so we select some demonstration projects that allow both Australian scientists and overseas scientists to really see their benefit. We were recently involved with a de novo sequencing project on coral. We felt it was vital for us get our hands well and truly into de novo sequencing to understand what combination of short reads and longer fragment reads or mate pairs was appropriate in order to start the Australian push for de novo sequencing. 

Coral was chosen for a number of reasons, particularly for its environmental importance, but also because there are a great collection of coral researchers already in the country who are very excited to get some sequence. The first round of data has been generated. We've put together a collaborative group of bioinformaticians because we also feel it's important for us to help the bioinformatics community learn about these data sets. Nothing is kept confidential and if things work great, but if we try a particular approach and it needs to be modified, then the whole group learns from doing that.

 

How did AGRF come to use Illumina technology?

Our first introduction was certainly around the expression array phenomenon, and then later added SNP genotyping services. We'd been using other technology initially, but found that Illumina had a high-quality product. Illumina had innovative thoughts about design and the best way to get a high-quality answer from the technology. Within the last two years, we got started with the Genome Analyzer while dealing with the scientists around the country who wanted to get a feel for what next-generation sequencing (using short reads) could do for them. I think next-generation sequencing has just started to make people think about how to do science differently. It addresses everything from a full systems biology approach to those who just want to get to a particular point that they never thought they could get to before. We are starting to see people thinking—not just big science, but about science they didn't think they could possibly do before and that's a really fun time in genomics.

Are you seeing any trends in integrated array- and sequencing-based approaches?

From the perspective in which we work, we certainly see that there's a great synergy, a really important marriage in there to start off with. For species that haven't had much polymorphism detection work done on them, to be able to sequence the genome using the Illumina platform, and then look across different species for the polymorphic variants to build onto customized chips is so empowering for the community now and so necessary. There are so many questions that people want to ask about important biological traits in animal populations that leaving things for too long really puts you well behind where the technology is allowing you to get now.

 

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