Whole genome sequencing services without compromise

The Genome Network

The Illumina Genome Network links researchers interested in conducting large whole human genome sequencing projects with leading institutes worldwide that provide highly economical and rapid turnaround access to Illumina sequencing. Comprised of CSPro™-certified organizations with proven expertise in generating high-quality, economical human genome data, the Illumina Genome Network enables researchers to complete their genome sequencing projects rapidly and confidently. Highest-quality data with fast turnaround times: that is the promise of the Illumina Genome Network.

 

Entrust your study to some of the most recognized names in sequencing, all of whom rely upon the trusted and proven Illumina technology platform. Leverage their expertise to get fast access to comprehensive whole-genome assemblies, including:

  • High quality variant calling of single nucleotide polymorphisms (SNPs) and insertions and deletions (Indels)
  • Ability to identify Copy Number Variations (CNV) and other structural rearrangements
  • Access to a comprehensive set of in-house and third-party analysis tools designed to support the system with the largest installed base in the industry
  • Ability to reanalyze data sets over time

You can choose your Illumina Genome Network partner and create service packages based on your individual study requirements. Flexible turnaround times and a range of value-added services are available to meet the unique needs of every study. It’s a quick and reliable way to get your sequencing study started on the Illumina sequencing technology platform.

To find out more information or get started today, please fill out the lead form.


IGN Data Sheet

The Genome Network Partners

Macrogen

Macrogen/Genomic Medicine Institute/Seoul National University
Rm 1002, 10th floor
World Merdian Venture Center
60-24, Gasan-dong, Geumchen-gu
Seoul, 153-781, South Korea

Phone: +82.2.2113.7000
Fax: +82.2.2113.7016
Email: chipinfo@macrogen.com
Web: www.macrogen.co.kr/kor/biochip/biochip_main.jsp

Genomic Medicine Institute (GMI) was founded in 1997 with the mission of providing Asian whole genome sequences and supporting the development of genomic technology for future personalized medicine and taking a role as the institute of excellence for Genomics in Korea.

NCGR (National Center for Genome Resources)

NCGR (National Center for Genome Resources)
2935 Rodeo Park Drive East
Santa Fe, New Mexico 87505

Contact: Dr. Ryan Kim, Genome Center Director
Phone: 1.505.995.4419
Fax: 1.505.995.4432
Email: seq@ncgr.org
Web: www.ncgr.org

NCGR, a non-profit research institute with extensive experience in Illumina DNA sequence data generation and analyses, provides sequencing, genotyping, and analysis services in a collaborative setting. Service offerings include whole-genome (including de novo), transcriptome, methylome, ChIP, smRNA, and exome (targeted or whole). Assistance with experimental design, custom data analysis, and visualization tools for de novo assembly, alignment, and variant detection are available. You can also contact us for Illumina genotyping services.

Service Details

When you send your project to a Genome Network Partner, you will receive whole human genome sequencing performed at 30X average coverage, from short-insert paired end reads, using a single library. Data analysis will be performed using CASAVA (consensus sequence from ELAND alignment, called SNPs, and small indels). The delivered data set will include reads and quality scores for further downstream analysis.

The following specifications are guaranteed for each sample:

  • Average autosomal fold coverage of 30X
  • Percent of non-N reference coverage greater than 94% for non-cancer samples
  • At least 3,000,000 SNPs detected for non-cancer samples
  • Genotype Concordance greater than 99% for non-cancer samples

Data will be delivered in standard formats for downstream visualization in Genome Studio or third-party open-source tools, as follows:

  • Processed data (aligned and sorted reads) in BAM format
  • Raw data (reads and quality scores) in a standard format
  • List of identified SNPs and indels (insertions/deletions) in text format
  • Genotype report

Premium data analysis packages are also available through select Genome Network Providers. Please send questions directly to your GN Partner.

The data will indicate which genomes meet and do not meet the following criteria:

  • Ratio of heterozygous sites between 1.3 and 1.9
  • Number of novel SNPs compared to dbSNP129/130 that are greater than 300,000
  • Transition:Transversion ratio between 1.95 and 2.05 (M. Nei, Molecular Evolutionary Genetics, p. 28)
  • Percent of heterozygous sites relative to NCBI length between 0.6x10-3 – 0.8x10-3

These criteria were determined by looking at meta-statistics for the sequencing results from ~40 genome runs over three locations as well as expected results from literature. Genomes that do not meet all of these criteria are deemed invalid and will either be resequenced or have any deviations thoroughly investigated, explained, and approved by the laboratory director.

Interested in Genome Network Services?

  1. Fill out web form to submit your project for the Genome Network (GN)
  2. Illumina provides a quote to you
    (per your submitted project specifications, including a list of GN Partners who can provide the service)
  3. You confirm acceptance of subcontracting terms and rank GN Partners of choice, including Illumina FastTrack Services
  4. Illumina approaches the GN Partners according to your ranking order
  5. GN Partner accepts your project
  6. You send samples to the GN Partner to begin project
  7. GN Partner notifies Illumina via email that data has been delivered to you to complete project
  8. You receive an invoice from Illumina