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Multiplexing Sample Preparation Oligonucleotide Kit Support
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Catalog IDs: PE-400-1001
The Multiplexing Sample Preparation protocol prepares libraries of DNA fragments for multiplexed paired-end or single-read sequencing. Adapter sequences are added onto the ends of DNA fragments.
Features
- Uses a novel set of oligonucleotides, but is otherwise the same as the standard paired-end sample preparation procedure
- A six-base sequence index is introduced into the adapter at the PCR stage of sample preparation
- Kit contains 12 index primers designed so that there are no more than three positions of agreement between any two indexes
- If there is one error in the index, the correct index can be recovered
- Designed for preparing samples for paired-end multiplex sequencing, however, these samples are also compatible with single-read multiplex sequencing
Libraries prepared with the Multiplexing Sample Preparation Oligonucleotide Kit can be sequenced on the HiSeq 2000, HiSeq 1000, HiScanSQ, or Genome Analyzer.
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