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BeadXpress
Custom Low- to Mid-plex Genotyping
For profiling and validation studies requiring low to mid-plex genotyping, Illumina provides customized solutions that allow researchers to optimize the number of loci per sample and throughput level to best suit their study goals. Custom assay panels can be easily developed for any species and deployed with either BeadArray or Veracode technology.
GoldenGate Custom Genotyping on BeadArray
Interrogate 96, or from 384 to 3,072 SNP loci simultaneously with the proven GoldenGate Genotyping Assay. The protocol can be performed manually or can be easily automated, with a streamlined workflow that allows a single technician to generate over 300,000 genotypes in just six hands-on hours. Custom Panels can be designed for many species with user-defined content.
GoldenGate Custom Genotyping on Veracode
Genotyping with VeraCode technology is available with three robust and customizable solutions that facilitate low- to mid-plex assays.
- GoldenGate Kits (48-, 96-, 144-, 192-, and 384-plex)
- Universal Capture Bead Sets (1-144-plex)
- Carboxyl Bead Sets (1-48-plex)
The GoldenGate Genotyping Assay is available in 48-384 multiplex kits for the VeraCode platform, providing a flexible customized solution for mid-level multiplexed assays. Universal Capture Bead Sets are an optimal solution for allele-specific primer extension (ASPE) assays. Carboxyl Bead Sets enable the immobilization of target-specific capture sequences on a coded carboxyl surface.
Custom Low- to Mid-plex Methylation Analysis
For methylation profiling studies requiring low- to mid-plex analysis, Illumina provides a customized solution that allows researchers to target CpG loci within genes or regions of interest. With the proven VeraCode GoldenGate Methylation Assay, customized probes can be easily developed for virtually any experimental design.
GoldenGate Customized Methylation Panels on VeraCode
Methylation profiling with Veracode technology provides a simplified workflow, high-throughput sample processing, and assay flexibility. A customized panel of 96-384 CpG regions can be profiled simultaneously, and hundreds of samples can be read per day with the BeadXpress Reader. Individual bead types can be combined in unlimited combinations to support flexible assay design tailored to specific applications. Submit your desired list of CpG regions, and Illumina scientists will help you create successful custom content for multiplexed GoldenGate Methylation assays.
Protein Screening
VeraCode technology provides a simple and flexible solution for multiplexed protein assays. With VeraCode Carboxyl Bead Sets, any protein or peptide can be covalently attached to the highly stable microbead surface, enabling the development of standard "sandwich" immunoassays. The 48 unique Carboxyl bead types can be pooled in varying combinations to perform up to 48 immunoassays in a single reaction in a standard 96-well microplate.
Analytes can be labeled with standard fluorescent reporters such as phycoerythrin (R-PE), Cy3, Cy5, or AlexaFluor dyes. During analysis, the fluorescence and code inscription for each microbead are detected by the BeadXpress Reader.
It has been demonstrated that the BeadXpress Reader can detect protein concentrations as low as 10 pg/ml in a multiplexed VeraCode Carboxyl bead assay.
The VeraScan Software that comes with the BeadXpress Reader System provides users the flexibility they need to customize scanning protocols. Illumina's BeadStudio software provides users a forum for simple to complex data analysis that can be easily exported to third-party data analysis programs.
Learn more about Carboxyl Bead Sets.
At Illumina, our goal is to apply innovative technologies and revolutionary assays to the analysis of genetic variation and function, making studies possible that were not even imaginable just a few years ago. These studies will help make the realization of personalized medicine possible. With such rapid advances in technology taking place, it is mission critical to have solutions that are not only innovative, but flexible, scalable, and complete with industry-leading support and service. As a global company that places high value on collaborative interactions, rapid delivery of solutions, and prioritizing the needs of its customers, we strive to meet this challenge. Illumina's innovative, array-based solutions for DNA, RNA, and protein analysis serve as tools for disease research, drug development, and the development of molecular tests in the clinic.