The TruSeq PE Cluster Kit v2-cBot-GA provides reagents for the cBot cluster amplification system. DNA library samples are bound to complementary adapter oligos grafted on the surface of the flow cell. The templates are copied from the hybridized primer by 3' extension using a high fidelity DNA polymerase. These copies are isothermally amplified to create clonal clusters of ~1,000 copies each, ready for sequencing.

Cluster generation reagents are provided in a pre-mixed, 96-well plate format that requires minimal reagent preparation. The kit also includes reagents for cluster resynthesis of the reverse strand. The kit also includes reagents for cluster resynthesis of the reverse strand, regenerated by bridge amplification within the paired-end flow cell with the Paired-End Module. After resynthesis of the reverse strand, the original forward strand is cleaved and the reverse strand is sequenced for the second read.

The TruSeq SR Cluster Kit v2-cBot-GA contains cluster generation reagents for the cBot cluster amplification system in a pre-mixed, 96-well plate format that requires minimal reagent preparation. DNA library samples are bound to complementary adapter oligos grafted on the surface of the flow cell. The templates are copied from the hybridized primer by 3' extension using a high fidelity DNA polymerase. These copies are isothermally amplified to create clonal clusters of ~1,000 copies each, ready for sequencing.

The TruSeq SR Cluster Kit v3-cBot-HS contains cluster generation reagents for the cBot cluster amplification system in a pre-mixed, 96-well plate format that requires minimal reagent preparation. DNA library samples are bound to complementary adapter oligos grafted on the surface of the flow cell. The templates are copied from the hybridized primer by 3' extension using a high fidelity DNA polymerase. These copies are isothermally amplified to create clonal clusters of ~1,000 copies each, ready for sequencing.

The TruSeq SBS Kit v3-HS reagents reduce hands-on preparation time to two minutes.

For single reads, it enables up to 600 Gb of output per run on the HiSeq 2000 or up to 150 Gb of output per run on the HiScanSQ, when used in conjunction with the TruSeq SR Cluster Kit v3-cBot-HS.

For paired-end reads, it enables up to 150 Gb of output per run on the HiSeq 2000 or up to 75 Gb of output per run on the HiScanSQ, when used in conjunction with the TruSeq PE Cluster Kit v3-cBot-HS.

Note: This kit is NOT compatible with the Genome Analyzer.

Chromatin immunoprecipitation (ChIP) is a powerful method to selectively enrich for DNA sequences bound by a particular protein in living cells. ChIP-Seq on Illumina sequencing systems supports virtually unconstrained selection of any ChIP-able protein and/or modification to be studied. These include transcription factors, polymerases and transcriptional machinery, structural proteins, protein modifications, and DNA modifications. The ChIP-Seq DNA Sample Prep Kit is used to build DNA libraries for single-read sequencing.

The ChIP process enriches specific crosslinked DNA protein complexes using an antibody against a protein of interest. Unique oligonucleotide adapters are then added to the small stretches of DNA that were bound to the protein of interest to enable massively parallel sequencing. Following a short PCR enrichment, the library is ready to load on cBot or Cluster Station for cluster generation.

Following DNA fragmentation, 2-5 Kb fragments are end-repaired with biotin labeled dNTPs. The DNA fragments are circularized, and non-circularized DNA is removed by digestion. Circular DNA is fragmented and biotin labels of the fragments (corresponding to the ends of the original DNA ligated together) are affinity purified. Purified fragments are end-repaired and ligated to Illumina Paired-End sequencing adapters.

Additional sequences complementary to the flow cell oligonucleotides are added to the adapter sequence with tailed PCR primers. The final prepared libraries consist of short fragments made up of two DNA segments that were originally separated by several kilobases. These libraries are ready to load on cBot or the Cluster Station for cluster generation.

The kit selectively targets microRNA, or other polycistronic RNAs that have been processed by DICER or similar nucleases, which provide a distinct molecular signature. The RNA fragments are ligated to Illumina adapters followed by reverse transcription and PCR amplification. These libraries are ready to load on cBot or the Cluster Station for cluster generation.

Ends are repaired with a combination of fill-in reactions and exonuclease activity to produce blunt ends. An 'A'- base is added to the blunt ends followed by ligation to Illumina Paired-End Sequencing adapters. These adapters contain two unique sequencing primer hybridization sites.

Additional sequences complementary to the oligonucleotides in the flow cell are added to the adapter sequences with tailed PCR primers, followed by gel-based size selection and purification. These libraries are ready to load on cBot or the Cluster Station for cluster generation.

The TruSeq PE Cluster Kit v3-cBot-HS provides reagents for the cBot cluster amplification system. DNA library samples are bound to complementary adapter oligos grafted on the surface of the flow cell. The templates are copied from the hybridized primer by 3' extension using a high fidelity DNA polymerase. These copies are isothermally amplified to create clonal clusters of ~1,000 copies each, ready for sequencing.

Cluster generation reagents are provided in a pre-mixed, 96-well plate format that requires minimal reagent preparation. The kit also includes reagents for cluster resynthesis of the reverse strand, regenerated by bridge amplification within the paired-end flow cell. After resynthesis of the reverse strand, the original forward strand is cleaved and the reverse strand is sequenced for the second read.