Eco Real-Time PCR System

Thermal system

Single Peltier-based system

Block format

48-well block

Consumables

48-well Eco plates and optical Eco adhesive seals

Accessories

Eco sample loading dock

Sample volumes

5–20µl validated (optimized for standard 20µl protocols)

Average ramp rate

5.5°C/sec

Temperature range

25–100°C

Temperature uniformity

± 0.1°C

Optical system

LED excitation (452–486 nm and 542–582 nm), four emission filters (505–545 nm, 562–596 nm, 604–644 nm, and 665–705 nm) and CCD camera

Calibrated dyes at shipment

SYBR, FAM, HEX, VIC, ROX, Cy5: factory-calibrated. Additional dyes within wavelength range of Eco filters are supported with no additional calibration required for implementation

Data collection

Data collected in all four filters for all wells regardless of plate setup; plate setup for data analysis can be altered after run completion.

Melt curve analysis supports continuous data acquisition in a single channel, providing increased data point collection and reduced run times

Real-Time PCR run time (40 cycles)

Less than 40 minutes 

Electrical

Voltage: 120–240 VAC=10%
Nominal current draw: 8A
Frequency: 50/60 Hz=1%
Peak Power 500VA, typical power is 180VA

Software

Eco System Software supports all chemistries and a variety of applications, including Absolute Quantification, Relative Quantification, Allelic Discrimination, and High Resolution Melt curve analysis (HRM)

Service contract

12-month service contract (includes parts and labor)

Instrument dimensions

12.6" closed (height)
14.5" open (height)
13.6" (depth)
12.2" (width)

32 cm closed (height)
36.8 cm open (height)
34.5 cm (depth)
31 cm (width)

Weight

30 lbs (13.6 Kg)

Sensitivity

1 copy

Dynamic range

9 logs linear range

Precision

Discriminates between 5,000 and 10,000 template copies with more than 99% confidence