The TruSeq PE Cluster Kit v5-CS-GA provides reagents for amplification on the Cluster Station. DNA library samples are bound to complementary adapter oligos grafted on the surface of the flow cell. The templates are copied from the hybridized primer by 3' extension using a high fidelity DNA polymerase. These copies are isothermally amplified to create clonal clusters of ~1,000 copies each, ready for sequencing.

The master-mixed reagents provide a streamlined workflow with a total of 20 minutes hands-on time for reagent preparation and setup. The kit also includes reagents for cluster resynthesis of the reverse strand, regenerated by bridge amplification within the paired-end flow cell with the Paired-End Module. After resynthesis of the reverse strand, the original forward strand is cleaved and the reverse strand is sequenced for the second read.

The TruSeq SR Cluster Kit v5-CS-GA provides master-mixed reagents for cluster generation using the Cluster Station. DNA library samples are bound to complementary adapter oligos grafted on the surface of the flow cell. The templates are copied from the hybridized primer by 3' extension using a high fidelity DNA polymerase. These copies are isothermally amplified to create clonal clusters of ~1,000 copies each, ready for sequencing.

Ready-to-load reagents for use in the Genome Analyzer systems reduce hands-on preparation time to ten minutes. At read lengths of up to 100 bp, 80% of bases have quality scores (Q) in excess of 30, maximizing the ability to accurately characterize the genome and minimizing errors. The chemistry also delivers the highest yield of perfect reads. At read lengths of up to 100 bp, greater than 85% of reads map with no errors, giving scientists confidence to detect even rare events at lower coverage levels.

The TruSeq PE Cluster Kit v2-cBot-GA provides reagents for the cBot cluster amplification system. DNA library samples are bound to complementary adapter oligos grafted on the surface of the flow cell. The templates are copied from the hybridized primer by 3' extension using a high fidelity DNA polymerase. These copies are isothermally amplified to create clonal clusters of ~1,000 copies each, ready for sequencing.

Cluster generation reagents are provided in a pre-mixed, 96-well plate format that requires minimal reagent preparation. The kit also includes reagents for cluster resynthesis of the reverse strand. The kit also includes reagents for cluster resynthesis of the reverse strand, regenerated by bridge amplification within the paired-end flow cell with the Paired-End Module. After resynthesis of the reverse strand, the original forward strand is cleaved and the reverse strand is sequenced for the second read.

The TruSeq SR Cluster Kit v2-cBot-GA contains cluster generation reagents for the cBot cluster amplification system in a pre-mixed, 96-well plate format that requires minimal reagent preparation. DNA library samples are bound to complementary adapter oligos grafted on the surface of the flow cell. The templates are copied from the hybridized primer by 3' extension using a high fidelity DNA polymerase. These copies are isothermally amplified to create clonal clusters of ~1,000 copies each, ready for sequencing.

The TruSeq Exome Enrichment Kit supports cost-effective, scalable exome sequencing studies, offering pre-enrichment sample pooling and the most comprehensive exome coverage, highest uniformity, and lowest DNA input requirement. Master-mixed reagents are coupled with plate-based processing for up to 96 samples and volumes are optimized for liquid handling robots, making the process automation-friendly for even higher throughput.

Prior to exome enrichment, libraries are prepared using the TruSeq DNA Sample Prep Kit, which provides a robust indexing solution for each DNA sample, enabling multi-sample pooling of up to six samples in a single enrichment reaction. Multi-sample pooling dramatically reduces hands-on time compared to other available methods, making large high-throughput studies feasible and economical.

*Limited-time offer
TruSeq Exome Enrichment—starting at just $49 USD per sample! Contact your sales representative to order today.

*Pricing valid for US customers only.  Promotional pricing for customers outside the US and in distributor countries will vary.

Featured Researchers

“HiSeq has been a game changer for us. A project that would have taken us a year, now takes one week.”

—GRAHAM WILEY, Ph.D.
Associate Research Scientist, Arthritis & Clinical Immunology Research Program,
Oklahoma Medical Research Foundation

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“With the HiSeq, we can sequence deeply enough that we’re not only able to discover mutations, but we can also find very rare mutations.”

—SETH CROSBY, M.D.
Director, Genome Technology Access Center,
Washington University at St. Louis

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Workflow improvements enable streamlined sample preparation, allowing economical studies covering all small RNA transcripts in any species. Illumina's unique combination of long and short reads, single and paired-end sequencing, and capacity for tens of millions to billions of reads per run enables researchers to:

• Annotate coding SNPs
• Discover transcript isoforms
• Identify regulatory RNAs
• Characterize splice junctions
• Determine the relative abundance of transcripts

Learn more about TruSeq technology.

Custom oligonucleotide probes are first designed and ordered in DesignStudio. The TruSeq Custom Oligo Set consists of 2,500 to 67,200 attempted custom oligos. TruSeq Custom Enrichment is fully integrated with TruSeq DNA Sample Preparation Kits for an optimized, gel-free workflow.

Based on the total number of samples requested, the TruSeq Custom Oligo Set  includes sufficient 4-, 8- , 24-, or 96-reaction TruSeq Custom Enrichment reagent kits. Through pre-enrichment sample pooling of up to 12 samples, the 4-reaction kit supports 48 samples, the 8-reaction kit supports 96 samples, the 24-reaction kit supports  288 samples, and the  96-reaction kit supports  1,152 samples.

*Limited-time offer

Get a 25% discount on the TruSeq Custom Enrichment 8-reaction, 96 Sample Kit. Contact your sales representative to order today.
*Promotional pricing for customers outside the US, Europe, and in distributor countries (including European countries covered by a distributor) may vary. No quantity restrictions. Not valid with other discounts.

Start a new TruSeq Custom Enrichment project »
Learn more about DesignStudio »

Learn more about TruSeq technology.

The industry's fastest sample prep.

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Workflow shown using the Nextera Sample Preparation Kit, and 1 x 36 bp sequencing on MiSeq system.

With Nextera technology, DNA is simultaneously fragmented and tagged with sequencing adapters in a single step, using standard lab equipment. Ideal for precious samples available in limited quantity, the protocol requires only 50 ng of DNA input. Libraries prepared with Nextera kits are compatible with all Illumina sequencers.

The TruSeq Dual Index Sequencing Primer Box is required for single-read and paired-end sequencing of Nextera libraries on HiSeq 1000/2000, HiScanSQ, and Genome Analyzer IIx instruments. These primers are required for non-indexed, single-indexed, and dual-indexed Nextera libraries.

The kit selectively targets microRNA, or other polycistronic RNAs that have been processed by DICER or similar nucleases, which provide a distinct molecular signature. The RNA fragments are ligated to Illumina adapters followed by reverse transcription and PCR amplification. These libraries are ready to load on cBot or the Cluster Station for cluster generation.

Master-mixed reagents eliminate the majority of pipetting steps and reduce the amount of clean-up, as compared to previous methods, minimizing hands-on time. This results in economical, high-throughput RNA sequencing studies achieved with the easiest-to-use sample preparation workflow offered by any NGS platform.

Learn more about TruSeq technology.

Master-mixed reagents eliminate the majority of pipetting steps and reduce the amount of clean-up, as compared to previous methods, minimizing hands-on time. This results in economical, high-throughput DNA sequencing studies achieved with the easiest-to-use sample preparation workflow offered by any sequencing platform.

• The TruSeq DNA v2 Sample Preparation kit includes a gel-free protocol optimized for use with the TruSeq Exome Enrichment Kit and the TruSeq Custom Enrichment Kit

Learn more about TruSeq technology.

Available for single- or paired-end read sequencing, each box includes sufficient reagents for processing a single flow cell.

Ends are repaired with a combination of fill-in reactions and exonuclease activity to produce blunt ends. An 'A'- base is added to the blunt ends followed by ligation to Illumina Paired-End Sequencing adapters. These adapters contain two unique sequencing primer hybridization sites.

Additional sequences complementary to the oligonucleotides in the flow cell are added to the adapter sequences with tailed PCR primers, followed by gel-based size selection and purification. These libraries are ready to load on cBot or the Cluster Station for cluster generation.