Illumina HiSeq 2000

HiSeq 2000


Applications: DNA Sequencing, Gene Regulation Analysis, Sequencing-Based Transcriptome Analysis, SNP Discovery and Structural Variation Analysis, Cytogenetic Analysis, DNA-Protein Interaction Analysis (ChIP-Seq), Sequencing-Based Methylation Analysis, Small RNA Discovery and Analysis


The HiSeq 2000 sequencing system offers unprecedented output and a breakthrough user experience. Leveraging Illumina's proven and widely-adopted, reversible terminator-based sequencing by synthesis chemistry in combination with innovative engineering, HiSeq 2000 delivers the industry's highest sequencing output and fastest data generation rate. Human interaction design features and the easiest sequencing workflow set a new standard for simplicity and user experience.
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System Description

With innovative design features, HiSeq 2000 is the easiest to use next-generation sequencing system.

  • Flow cells are easily loaded on the vacuum-controlled loading dock.
  • Pre-configured, plug-and-play reagents sufficient for up to 200 cycles drop into racks in the machine's chiller compartment, requiring only two minutes of hands-on time.
  • A simple touch screen user interface, including on-screen, step-by-step instructions with embedded multimedia help, simplifies run setup.
  • Real-time progress indicators provide at-a-glance status, and remote monitoring allows a single user to check progress on multiple systems from any browser or internet-enabled phone.
  • HiSeq 2000 can be operated in single or dual flow cell mode, offering unmatched experimental flexibility and instrument scalability.
  • Independently-operable flow cells allow applications requiring different read lengths to run simultaneously.
  • Illumina's efficient and scalable data analysis solution for transforming billions of bases of raw sequencing data into publishable, biologically meaningful results is included.

 

HIGHLIGHTS

Unprecedented Output

Up to 200 Gb per run, 2 x 100 bp read length, up to 25 Gb per day, two billion paired-end reads/run.

Breakthrough User Experience

Pre-configured, plug-and-play reagents, simple flow cell loading, touch screen-enabled user interface for step-by-step run setup, and integrated paired-end fluidics.

Unmatched Cost-Effectiveness

In a single run, sequence two human genomes at ~30x coverage for less than $10,000 (USD) per genome, or perform 200 gene expression profiles for less than $200 per sample.

 

Sequence at a Scale Never Before Possible

HiSeq 2000 is the first commercially available sequencer to enable researchers to obtain ~30x coverage of two human genomes in a single run for under $10,000 (USD)* per sample.

 

HiSeq 2000 makes it possible for individual labs to take on the largest and most complex sequencing studies at the lowest cost. The ability to process larger numbers of samples and to decode larger and more complex genomes means that virtually any sequencing project is now within reach.

 

*Reagent costs at list price.

Upcoming Events

European Society of Human Genetics (ESHG)
June 12-15, 2010
Gothenburg, Sweden

Workflow & Specs

HiSeq 2000 Preliminary Performance SpecificationsHiSeq 2000 Workflow

Options & Accessories

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HiSeq 2000 Kits

cBot Single Read Cluster Generation Kit

cBot Single Read Cluster Generation Kit

The cBot Single Read Cluster Generation Kit provides reagents that bind DNA library samples to complementary adapter oligonucleotides grafted on the flow cell surface. The pre-mixed, 96-well plate format reagents in this kit provide a streamlined workflow with no reagent preparation and a total of 10 minutes hands-on time.

Additional Details

GD-300-1001
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cBot Paired-End Cluster Generation Kit

cBot Paired-End Cluster Generation Kit

The cBot Paired-End Cluster Generation Kit provides reagents that bind samples to complementary adapter oligos on the flow cell. These enable copying of the DNA strand after the first sequencing run; the copied strand is used to sequence the other fragment end. The cBot amplifies attached DNA fragments to clonal clusters of ~1000 copies each.

Additional Details

PE-300-1001
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HiSeq 2000 Paired-End Cluster Generation Kit

HiSeq 2000 Paired-End Cluster Generation Kit

The HiSeq Paired-End Cluster Generation Kit provides reagents that bind samples to complementary adapter oligos on the flow cell. These enable copying of the DNA strand after the first sequencing run; the copied strand is used to sequence the other fragment end. cBot amplifies attached DNA fragments to clonal clusters of ~1000 copies each.

Additional Details

PE-401-1001
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HiSeq 2000 Single-Read Cluster Generation Kit

HiSeq 2000 Single-Read Cluster Generation Kit

The HiSeq Single-Read Cluster Generation Kit provides reagents that bind samples to complementary adapter oligos on the flow cell.

Additional Details

GD-401-1001
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Small RNA Sample Prep Kit

Small RNA Sample Prep Kit

With Small RNA Discovery and Analysis, Illumina scientists have designed a sample preparation protocol that enables sequencing of of known and novel microRNAs as well as other non-coding RNAs, such as piwi-interacting RNAs and siRNAs. Sequencing-based Small RNA Sample Prep Kits offer researchers the flexibility to select the length of the small RNA they wish to investigate, enabling size-focused or broad size range investigation of different classes of small RNA on one universal platform.

Additional Details

FC-102-1009
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Paired-End DNA Sample Prep Kit V1

Paired-End DNA Sample Prep Kit V1

The Paired-End DNA Sample Prep Kit helps build DNA libraries for paired-end sequencing with insert sizes from 200-500 bp. Preparation includes introduction of two unique sequencing primer binding sites and adapter sequences to the anti-parallel strands of the polished whole-genome DNA fragments followed by gel-based size selection and purification.

Additional Details

PE-102-1001
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mRNA-Seq 8-Sample Prep Kit

mRNA-Seq 8-Sample Prep Kit

The mRNA-Seq 8-Sample Prep Kit is used to build DNA libraries for single-read and paired-end sequencing on the Genome Analyzer. Sample preparation is straightforward, using standard molecular biology techniques and requiring minimal hands-on time. mRNA template libraries are sequenced just as any DNA sample using Illumina Sequencing.

Additional Details

RS-100-0801
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Multiplexing Sample Preparation Oligonucleotide Kit

Multiplexing Sample Preparation Oligonucleotide Kit

The Multiplexing Sample Preparation Oligonucleotide Kit contains 12 unique oligonucleotides to “tag” libraries for pooling in one flow cell lane. Up to 96 samples can be sequenced on a single flow cell using the Genome Analyzer. The multiplex sequencing process is fully automated using the Cluster Station, Genome Analyzer, and Paired-End Module.

Additional Details

PE-400-1001
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Mate Pair Library Prep Kit

Mate Pair Library Prep Kit

The Mate Pair Library Prep Kit includes reagents needed to generate libraries with inserts from 2-5 kb in size. Prepared libraries consist of short fragments made up of two segments that were originally separated by several kilobases in the genome. Mate Pairs are sequenced using a two-adapter strategy identical to the paired-end sequencing method.

Additional Details

PE-112-1002
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Genomic DNA Sample Prep Kits

Genomic DNA Sample Prep Kits

Go from purified genomic DNA to a DNA library. Genomic DNA Sample Prep Kits include reagents to build DNA libraries for single-read sequencing. Random size–selected fragments of DNA are created, polished, and prepared for the addition of unique adapters to fragment ends. Following a short PCR enrichment, the library is ready to load.

Additional Details

FC-102-1001
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Tag Profiling Sample Prep Kits

Tag Profiling Sample Prep Kits

Tag Profiling Sample Prep Kits are used to build constructs comprised of a unique, positionally known 20- or 21-base pair cDNA tag with defined adapters attached to both ends. Access to any mRNA is through two restriction enzyme tag construction options. Kits are available complete or containing only the required oligonucleotides.

Additional Details

FC-102-1005
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ChIP-Seq DNA Sample Prep Kit

ChIP-Seq DNA Sample Prep Kit

Chromatin immunoprecipitation (ChIP) selectively finds DNA sequences bound by a particular protein. The ChIP-Seq DNA Sample Prep Kit helps build DNA libraries. The ChIP process enriches specific crosslinked DNA protein complexes using an antibody and unique oligonucleotide adapters added to small stretches of DNA bound to the protein of interest.

Additional Details

IP-102-1001
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Multiplexing Sequencing Primers and PhiX Control Kit V2

Multiplexing Sequencing Primers and PhiX Control Kit V2

The Multiplexing Sequencing Primers and PhiX Control Kit V2 is used to sequence samples tagged by using the Multiplexing Sample Preparation Oligonucleotide Kit and then amplified in the cBot or Cluster Station. Sequencing is fully automated on an Illumina sequencing system.

Additional Details

PE-400-2002
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TruSeq SBS V5 HS kits

TruSeq SBS V5 HS kits

TruSeq SBS V5 HS kits contain ready-to-load reagents for accurately determining the DNA sequence of each cluster on a flow cell using sequencing by synthesis technology on the HiSeq 2000 sequencing system.

Additional Details

FC-401-1001
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Software

Illumina proudly offers system and analysis software designed to maximize your data quality and throughput level. Each instrument includes system control software designed for optimal performance. Data can be analyzed using Illumina's user-friendly GenomeStudio software or a third-party package.

 

 

IDEA Challenge

Illumina has launched a program designed to challenge the scientific community to develop new and creative integrative visualization and data analysis techniques. Learn more about the iDEA Challenge.

Documentation

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Support

Illumina's Customer Solutions Team is dedicated to providing you with the resources you need to support your research.  Scientists specializing in Illumina's technologies, scientific applications, software, and hardware systems are available even before your first system purchase.  Field Service Engineers expertly install and qualify your instrument to guarantee a fully functional system.  Field Application Scientists offer extensive training, enabling labs to quickly become proficient at generating industry-leading data quality at unparalleled throughput levels.  Technical Support Scientists are just a phone call or email away and can help you plan your project, diagnose a problem, and interpret results.

We strive to continuously exceed your expectations, so that you can maximize the impact of Illumina technology in your lab.  For more information, visit our Support section  


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