Any application. Any study.
| HiSeq 2500 | HiSeq 2000 | HiSeq 1500 | HiSeq 1000 | |||
| Run Mode | High Output | Rapid Run | High Output | High Output | Rapid Run | High Output |
| Output (2 x 100 bp) |
600 Gb | 120 Gb | 600 Gb | 300 Gb | 60 Gb | 300 Gb |
| Run Time (2 x 100 bp) |
~11 days | ~27 hours | ~11 days | ~8.5 days | ~27 hours | ~8.5 days |
| Cluster Generation |
cBot | On board | cBot | cBot | On board | cBot |
| Paired-end Reads | 6 Billion | 1.2 Billion | 6 Billion | 3 Billion | 600 Million | 3 Billion |
| Single Reads |
3 Billion | 600 Million | 3 Billion | 1.5 Billion | 300 Million | 1.5 Billion |
| Maximum Read Length** |
2 × 100 bp | 2 × 150 bp | 2 × 100 bp | 2 × 100 bp | 2 × 150 bp | 2 × 100 bp |
| Bases Above Q30*** | > 85% (2 x 50 bp) > 80% (2 x 100 bp) |
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| **Paired 150-cycle runs are supported only in the rapid run mode. Total output generated with 2 × 150 bp run anticipated to be up to 90 Gb per flow cell. Run time is approximately 40 hours. ***Based on an Illumina PhiX library at supported cluster densities. |
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