Illumina SQ Module

SQ Module


Applications: Sequencing, DNA Sequencing, Gene Regulation Analysis, Sequencing-Based Transcriptome Analysis, SNP Genotyping and CNV Analysis, Whole-Genome Genotyping and Copy Number Analysis


The SQ Module allows integration of the high-throughput power of genotyping and gene expression arrays with the flexibility and resolution of next-generation sequencing. It is an add-on device that, in concert with the Genome Analyzer Cluster Station, enables sequencing by synthesis on an existing iScan System. More...


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System Description

The SQ Module provides any lab that has traditionally run microarray assays an economical and convenient gateway to next-generation sequencing. The streamlined workflow ensures convenient operation and reproducible results with minimal user intervention.

The SQ Module enables resequencing of small genomes or targeted genomic regions, a deeper understanding of the transcriptome with mRNA-Seq, discovery and profiling of miRNA expression, characterization of genome-wide DNA-protein interaction sites with ChIP-Seq, and validation of array-based expression and splice variant data, and allows identification of target SNPs from sequencing experiments to design iSelect custom genotyping panels for any species and any study.

  • The SQ Module provides flexibility for paired-end reads, multiplexing (up to 12 samples per lane; 96 samples per flow cell), and customizable read lengths for any experimental design.
  • To perform sequencing on the iScan System, a Sequencing Module, Cluster Station, and data analysis tools are required.

HIGHLIGHTS

Integrated

Seamlessly combine array and sequencing assays to enable an unmatched range of applications

Flexible

Easily transition between array and sequencing assays

Cost-effective

Conveniently and economically add next-generation sequencing capabilities to your research

High-quality

Reliably generate the highest raw read accuracy and maximal output of perfect reads

Workflow and Specs


Workflow

Streamlined microarray and sequencing workflows allow you to quickly generate high-quality data, conveniently and reliably


Assays/Protocols

BeadArray: Infinium HD, DASL, Direct Hyb
Sequencing: Single-Read Sequencing, Paired-End Sequencing, Multiplex Sequencing, Mate-Pair Library Sequencing, ChIP-Seq Sequencing, mRNA-Seq, Small RNA Discovery and Analysis

iScan Specifications
Field of View 1 mm wide
Pixel Resolution 0.54 micron
Laser Excitation 532 nm and 658 nm dual-laser excitation
Emission Spectra 550-600 nm and 677-697 nm
Image Registration Automatic
Data Extraction Automatic
Image File Output TIFF, PNG, or JPG data file output with automatic image quality analysis (uncompressed or compressed)
Data File Output IDAT (Illumina proprietary format)
Detector Analog-to-Digital Converter 16-bit
Limit of Detection 5 fluor/µm2 (Cy3 or Cy5)
Input Voltage 100-240V AC 50/60 Hz, single phase
Input Power 3A (110V); 1.5A (220V)
Dimensions 52 cm (width) × 45 cm (height) × 66 cm (depth)
Dimensions of Air Table 61 cm (width) × 6 cm (height) × 69 cm (depth)
Weight 65 kg
iScanSQ Specifications
Width 61 cm (24 in)
Height 76 cm (30 in)
Depth 71 cm (28 in)
Weight 50 kg (110 lbs)
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SQ Module Kits

Illumina Sequencing Kits v4

Illumina Sequencing Kits v4

The Illumina Sequencing Kit v4 contains ready-to-load reagents for accurately determining the DNA sequence of each cluster on a flow cell using Sequencing by Synthesis technology and the Genome Analyzer. The improved chemistry of this kit enable researchers to conduct much longer, high-accuracy, sequencing runs than previous versions.

Additional Details

FC-104-4001
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Small RNA Sample Prep Kit

Small RNA Sample Prep Kit

With Small RNA Discovery and Analysis, Illumina scientists have designed a sample preparation protocol that enables sequencing of of known and novel microRNAs as well as other non-coding RNAs, such as piwi-interacting RNAs and siRNAs. Sequencing-based Small RNA Sample Prep Kits offer researchers the flexibility to select the length of the small RNA they wish to investigate, enabling size-focused or broad size range investigation of different classes of small RNA on one universal platform.

Additional Details

FC-102-1009
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Mate Pair Library Prep Kit

Mate Pair Library Prep Kit

The Mate Pair Library Prep Kit includes reagents needed to generate libraries with inserts from 2-5 kb in size. Prepared libraries consist of short fragments made up of two segments that were originally separated by several kilobases in the genome. Mate Pairs are sequenced using a two-adapter strategy identical to the paired-end sequencing method.

Additional Details

PE-112-1002
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Illumina Sequencing Kits v3

Illumina Sequencing Kits v3

The Illumina Sequencing Kit v3 contains ready-to-load reagents for accurately determining the DNA sequence of each cluster on a flow cell using Sequencing by Synthesis technology and the Genome Analyzer. The improved chemistry of this kit enable researchers to conduct much longer, high-accuracy, sequencing runs than previous versions.

Additional Details

FC-104-1001
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Multiplexing Sample Preparation Oligonucleotide Kit

Multiplexing Sample Preparation Oligonucleotide Kit

The Multiplexing Sample Preparation Oligonucleotide Kit contains 12 unique oligonucleotides to “tag” libraries for pooling in one flow cell lane. Up to 96 samples can be sequenced on a single flow cell using the Genome Analyzer. The multiplex sequencing process is fully automated using the Cluster Station, Genome Analyzer, and Paired-End Module.

Additional Details

PE-400-1001
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Multiplexing Sequencing Primers and PhiX Control Kit V1

Multiplexing Sequencing Primers and PhiX Control Kit V1

The Multiplexing Sequencing Primers and PhiX Control Kit V1 is used to sequence samples tagged by using the Multiplexing Sample Preparation Oligonucleotide Kit and then amplified in the Cluster Station. Sequencing is fully automated on the Genome Analyzer.

Additional Details

PE-400-1002
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Paired-End Cluster Generation Kit V2

Paired-End Cluster Generation Kit V2

The Paired-End Cluster Generation Kit V2 provides reagents that bind samples to complementary adapter oligos on the flow cell. These enable copying of the DNA strand after the first sequencing run; the copied strand is used to sequence the other fragment end. The Cluster Station amplifies attached DNA fragments to clonal clusters of around 1000 copies each.

Additional Details

PE-103-2001
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Standard Cluster Generation Kit v2

Standard Cluster Generation Kit v2

The Standard Cluster Generation Kit v2 provides ready-to-load reagents that bind DNA library samples to complementary adapter oligos grafted on the flow cell surface. The master-mixed reagents in this kit provide a streamlined workflow with a total of 10 minutes hands-on time for reagent preparation and setup.

Additional Details

FC-103-2001
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Paired-End DNA Sample Prep Kit V1

Paired-End DNA Sample Prep Kit V1

The Paired-End DNA Sample Prep Kit helps build DNA libraries for paired-end sequencing with insert sizes from 200-500 bp. Preparation includes introduction of two unique sequencing primer binding sites and adapter sequences to the anti-parallel strands of the polished whole-genome DNA fragments followed by gel-based size selection and purification.

Additional Details

PE-102-1001
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Standard Cluster Generation Kit v4

Standard Cluster Generation Kit v4

The Standard Cluster Generation Kit v4 provides ready-to-load reagents that bind DNA library samples to complementary adapter oligos grafted on the flow cell surface. The master-mixed reagents in this kit provide a streamlined workflow with a total of 10 minutes hands-on time for reagent preparation and setup.

Additional Details

GD-103-4001
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Paired-End Cluster Generation Kit V4

Paired-End Cluster Generation Kit V4

The Paired-End Cluster Generation Kit V4 provides reagents that bind samples to complementary adapter oligos on the flow cell. These enable copying of the DNA strand after the first sequencing run; the copied strand is used to sequence the other fragment end. The Cluster Station amplifies attached DNA fragments to clonal clusters of ~1000 copies each.

Additional Details

PE-203-4001
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cBot Paired-End Cluster Generation Kit

cBot Paired-End Cluster Generation Kit

The cBot Paired-End Cluster Generation Kit provides reagents that bind samples to complementary adapter oligos on the flow cell. These enable copying of the DNA strand after the first sequencing run; the copied strand is used to sequence the other fragment end. The cBot amplifies attached DNA fragments to clonal clusters of ~1000 copies each.

Additional Details

PE-300-1001
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cBot Single Read Cluster Generation Kit

cBot Single Read Cluster Generation Kit

The cBot Single Read Cluster Generation Kit provides reagents that bind DNA library samples to complementary adapter oligonucleotides grafted on the flow cell surface. The pre-mixed, 96-well plate format reagents in this kit provide a streamlined workflow with no reagent preparation and a total of 10 minutes hands-on time.

Additional Details

GD-300-1001
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Small RNA Cluster Generation Kits

Small RNA Cluster Generation Kits

For Digital Gene Expression (DGE): Small RNA Discovery and Analysis, the Small RNA Cluster Generation Kit is used to bind total RNA samples to complementary adapter oligos on a flow cell surface. The Cluster Station isothermally amplifies attached cDNA and resulting high-density template cluster array is sequenced by the Illumina Genome Analyzer.

Additional Details

FC-103-1008
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mRNA-Seq 8-Sample Prep Kit

mRNA-Seq 8-Sample Prep Kit

The mRNA-Seq 8-Sample Prep Kit is used to build DNA libraries for single-read and paired-end sequencing on the Genome Analyzer. Sample preparation is straightforward, using standard molecular biology techniques and requiring minimal hands-on time. mRNA template libraries are sequenced just as any DNA sample using Illumina Sequencing.

Additional Details

RS-100-0801
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ChIP-Seq DNA Sample Prep Kit

ChIP-Seq DNA Sample Prep Kit

Chromatin immunoprecipitation (ChIP) selectively finds DNA sequences bound by a particular protein. The ChIP-Seq DNA Sample Prep Kit helps build DNA libraries. The ChIP process enriches specific crosslinked DNA protein complexes using an antibody and unique oligonucleotide adapters added to small stretches of DNA bound to the protein of interest.

Additional Details

IP-102-1001
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Tag Profiling Sample Prep Kits

Tag Profiling Sample Prep Kits

Tag Profiling Sample Prep Kits are used to build constructs comprised of a unique, positionally known 20- or 21-base pair cDNA tag with defined adapters attached to both ends. Access to any mRNA is through two restriction enzyme tag construction options. Kits are available complete or containing only the required oligonucleotides.

Additional Details

FC-102-1005
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Tag Profiling Cluster Generation Kits

Tag Profiling Cluster Generation Kits

Tag Profiling Cluster Generation Kits are used with total RNA samples to bind the tag construct library to complementary adapter oligos grafted on the flow cell surface. The Cluster Station isothermally amplifies the attached cDNA constructs to create clonal clusters of roughly 1,000 copies each.

Additional Details

FC-103-1004
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Genomic DNA Sample Prep Kits

Genomic DNA Sample Prep Kits

Go from purified genomic DNA to a DNA library. Genomic DNA Sample Prep Kits include reagents to build DNA libraries for single-read sequencing. Random size–selected fragments of DNA are created, polished, and prepared for the addition of unique adapters to fragment ends. Following a short PCR enrichment, the library is ready to load.

Additional Details

FC-102-1001
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Software

Illumina proudly offers system and analysis software designed to maximize your data quality and throughput level. Each instrument includes system control software designed for optimal performance. Data can be analyzed using Illumina's user-friendly GenomeStudio software or a third-party package.

Documentation

Literature


Videos

  • iScan integrated array and sequencing video

    • Support

    Illumina's Customer Solutions Team is dedicated to providing you with the resources you need to support your research.  Scientists specializing in Illumina's technologies, scientific applications, software, and hardware systems are available even before your first system purchase.  Field Service Engineers expertly install and qualify your instrument to guarantee a fully functional system.  Field Application Scientists offer extensive training, enabling labs to quickly become proficient at generating industry-leading data quality at unparalleled throughput levels.  Technical Support Scientists are just a phone call or email away and can help you plan your project, diagnose a problem, and interpret results.

    We strive to continuously exceed your expectations, so that you can maximize the impact of Illumina technology in your lab.  For more information, visit our Support section  


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