Sequencing power for every scale

Find the sequencing system that’s right for your lab

Compare key specifications across the whole portfolio of Illumina sequencing systems. Understand the primary differences between the MiniSeq, MiSeq, NextSeq, HiSeq, and HiSeq X Series.


MiniSeq System


MiSeq Series


NextSeq Series


HiSeq Series


HiSeq X Series*

Key Methods Amplicon, targeted RNA, small RNA, and targeted gene panel sequencing. Small genome, amplicon, and targeted gene panel sequencing. Everyday exome, transcriptome, and targeted resequencing. Production-scale genome, exome, transcriptome sequencing, and more. Population- and production-scale whole-genome sequencing.
Maximum Output 7.5 Gb 15 Gb 120 Gb 1500 Gb 1800 Gb
Maximum Reads per Run 25 million 25 million 400 million 5 billion 6 billion
Maximum Read Length 2 × 150 bp 2 × 300 bp 2 × 150 bp 2 × 150 bp 2 × 150 bp
Run Time 4–24 hours 4–55 hours 12–30 hours <1–3.5 days (HiSeq 3000/HiSeq 4000)
7 hours–6 days (HiSeq 2500)
<3 days
Benchtop Sequencer Yes Yes Yes No No
System Versions

For help choosing a sequencing system:

* Specifications shown for an individual HiSeq X Sequencing System. HiSeq X is only available as part of the HiSeq X Five or HiSeq X Ten.
† For MiSeq V3 Kits only.