The cDNA-mediated Annealing, Selection, extension, and Ligation (DASL) Assay allows for RNA profiling of up to 1,536 targets using partially degraded RNA as found in formalin-fixed, paraffin-embedded (FFPE) samples. Using either the Universal-16 BeadChips or the Universal-96 Array Matrix formats for sample processing, the DASL Assay offers researchers the opportunity to analyze hundreds to thousands of RNA transcripts derived from newely or previously collected, preserved, samples.
The DASL Assay leverages the proven GoldenGate Assay protocol to provide high-quality data for an RNA-based application. Because the probe groups span only about 50 bases, partially degraded RNA can be used in the assay. The availability of additional, unique probe sequences per gene increases the sensitivity of the assay, allowing the quantitation of low-abundance transcripts, even in partially degraded samples.
DASL Assay Highlights