The cDNA-mediated Annealing, Selection, extension, and Ligation (DASL) Assay allows for expression profiling of partially degraded RNA, such as commonly found in formalin-fixed, paraffin-embedded (FFPE) samples. The Whole-Genome DASL HT Assay (>29,000 gene targets) offers researchers the opportunity to analyze hundreds to thousands of RNA transcripts in FFPE samples from as little as 50 ng total RNA, delivering highly reproducible results.
The DASL Assay leverages the unique PCR and labeling steps of the proven GoldenGate Assay protocol to provide high-quality data for an RNA-based application. Because the probe groups span only about 50 bases, partially degraded RNA can be used in the assay. The Whole-Genome DASL HT Assay utilizes the whole-genome probe set of Illumina’s BeadChips to provide highly reproducible expression profiles.
DASL Assay Highlights