The Infinium HD Assay leverages proven chemistry and a robust BeadChip platform to produce unrivaled data quality, superior call rates, and the most consistent reproducibility. From customized studies on targeted regions to large-scale genome-wide association studies, the flexible Infinium HD design offers a powerful solution for virtually any genetic analysis application.
Discover the technology: Watch the Infinium Arrays animation.
The Infinium HD Assay protocol allows for single tube sample preparation without PCR or ligation steps, significantly reducing labor and sample handling errors. The assay can be scaled to unlimited levels of multiplexing without compromising data quality, unlike many alternative PCR-dependent assays.
Genomic markers are interrogated though a two-step detection process. Carefully designed 50-mer probes selectively hybridize to the loci of interest, stopping one base before the interrogated marker. Marker specificity is conferred by enzymatic single-base extension to incorporate a labeled nucleotide.
Subsequent dual-color florescent staining allows the labeled nucleotide to be detected by Illumina’s iScan imaging system, which identifies both color and signal intensity. For genotyping assays, the red and green color signals specify each allele, where homozygotes are indicated by red/red or green/green signals, and heterozyotes are indicated by red/green (yellow) signals.