Infinium HD Assay

The Infinium HD Assay leverages proven chemistry and a robust BeadChip platform to produce unrivaled data quality, superior call rates, and the most consistent reproducibility. From customized studies on targeted regions to large-scale genome-wide association studies, the flexible Infinium HD design offers a powerful solution for virtually any genetic analysis application.

 

  • Infinium HD BeadChips
    The Illumina portfolio of Infinium HD BeadChips feature the most advanced genomic content available for genotyping, and copy number variation (CNV) and Cytogenetic analysis. Profile the human genome with 300,000 to nearly 1.2 million markers strategically-selected to provide dense coverage, few large gaps, and cutting-edge content associated with human disease.

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  • Infinium HD iSelect BeadChips
    Infinium HD iSelect BeadChips allow you to provide your own content for virtually any marker in the genome and any species of interest. Access the enhanced multiplexing capabilities of the Infinium HD Assay deployed on either the 12-sample format (60,801 to 200,000 attempted bead types), or the 24-sample format (3,000 to 68,000 attempted bead types). For array customization in human studies, add up to 60,800 markers to the standard whole-genome SNP genotyping and CNV identification content available on the semi-custom Human1M-Duo+ and HumanHap550-Quad+ BeadChips. Standard BeadChips are also available which feature focused content for agriculturally relevant species.

 

Convenient protocol
The Infinium HD Assay protocol allows for single tube sample preparation without PCR or ligation steps, significantly reducing labor and sample handling errors. The assay can be scaled to unlimited levels of multiplexing without compromising data quality, unlike many alternative PCR-dependent assays.


Powerful chemistry
Genomic markers are interrogated though a two-step detection process. Carefully designed 50-mer probes selectively hybridize to the loci of interest, stopping one base before the interrogated marker. Marker specificity is conferred by enzymatic single-base extension to incorporate a labeled nucleotide.

Subsequent dual-color florescent staining allows the labeled nucleotide to be detected by Illumina’s iScan imagining system, which identifies both color and signal intensity. For genotyping assays, the red and green color signals specify each allele, where homozygotes are indicated by red/red or green/green signals, and heterozyotes are indicated by red/green (yellow) signals.


Infinium Assay