Sequencing by Synthesis (SBS) Technology

Illumina’s sequencing technology, sequencing by synthesis (SBS), is the most successful and widely adopted NGS (next-generation sequencing) technology worldwide. Illumina sequencing reagents support massively parallel sequencing using a proprietary method that detects single bases as they are incorporated into growing DNA strands.

SBS Chemistry

A fluorescently labeled reversible terminator is imaged as each dNTP is added, and then cleaved to allow incorporation of the next base. Since all four reversible terminator-bound dNTPs are present during each sequencing cycle, natural competition minimizes incorporation bias. The end result is true base-by-base sequencing that enables the industry’s most accurate data for a broad range of applications.

Applications of SBS Technology

A wide array of sample preparation methods enable diverse sequencing applications, including: whole-genome sequencing, de novo sequencing, candidate region targeted resequencing, DNA sequencing, RNA sequencing (for applications such as transcriptome and small RNA analysis), methylation analysis, and protein-nucleic acid interaction analysis (ChIP-Seq).

Further Advantages of SBS Technology

SBS technology supports both single read and paired-end libraries. It is the only platform that offers a short-insert paired-end capability for high-resolution genome sequencing as well as long-insert paired-end reads using the same robust chemistry for efficient sequence assembly, de novo sequencing, large-scale structural variation detection, and more. The combination of short inserts and longer reads increase the ability to fully characterize any genome.

 

Systems

  HiSeq 2500
Production Power
  NextSeq 500
Flexible Power
  MiSeq
Focused Power

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See how researchers are using SBS technology to solve problems:

Cancer
Cancer
Microbiology
Microbiology
Agriculture
Agriculture
Forensics
Forensics
Genetic Disease
Genetic Disease