Illumina’s sequencing technology, sequencing by synthesis (SBS), is the most successful and widely adopted NGS (next-generation sequencing) technology worldwide. Illumina sequencing reagents support massively parallel sequencing using a proprietary method that detects single bases as they are incorporated into growing DNA strands.
A fluorescently labeled reversible terminator is imaged as each dNTP is added, and then cleaved to allow incorporation of the next base. Since all four reversible terminator-bound dNTPs are present during each sequencing cycle, natural competition minimizes incorporation bias. The end result is true base-by-base sequencing that enables the industry’s most accurate data for a broad range of applications.
A wide array of sample preparation methods enable diverse sequencing applications, including: whole-genome sequencing, de novo sequencing, candidate region targeted resequencing, DNA sequencing, RNA sequencing (for applications such as transcriptome and small RNA analysis), methylation analysis, and protein-nucleic acid interaction analysis (ChIP-Seq).
See how HiSeq X Ten breaks the $1000 genome barrier for human whole-genome sequencing.