Single-read Sequencing Assay

Single-read sequencing is the simplest way to utilize Illumina’s Genome Analyzer system whether you need to sequence an entire genome or a large candidate region.

By leveraging proprietary reversible terminator chemistry and a novel polymerase, this solution delivers unprecedented volumes of high-quality data, rapidly and economically. Reversible terminators avoid homopolymer sequencing errors seen in pyrosequencing or the high G-C bias observed in hybridization and ligation–based chemistries. The Genome AnalyzerIIx single-read technology can sequence up to eight samples, achieving 75 bp reads and up to 100 million reads per single-read run.

Single-Read Sequencing Highlights

  • Simple Library Preparation: Follows standard molecular biology methods; compatible with robotics
  • Low Input DNA Requirements: 100 ng–1 μg gDNA or cDNA
  • Economical: 1/100th the cost of traditional Sanger sequencing
  • Simplified Data Analysis: Higher quality sequence assemblies with short-insert libraries

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Single read sequencing