DNA methylation is an epigenetic modification known to have widespread effects on gene expression. Dysregulation of methylation has been linked to the etiology of common diseases such as cancer, MS, diabetes, and schizophrenia.

Illumina’s GoldenGate Assay for Methylation provides sensitive detection for profiling methylation status at single-site resolution. This robust assay deployed on VeraCode technology offers an economical and high-throughput solution for low- to mid-level multiplexing and presents an ideal platform for focused studies or biomarker validation.

A customized panel of 48-384 CpG regions can be profiled simultaneously, and hundreds of samples can be read per day with the BeadXpress Reader. Individual bead types can be combined in unlimited combinations to support flexible assay design tailored to specific applications. Submit your desired list of CpG regions, and Illumina scientists will help you create successful custom content for multiplexed GoldenGate Methylation assays.

VeraCode methylation highlights

• Flexible multiplex: target 48-384 CpG regions in a single reaction
• Specificity: obtain single-site resolution
• High sample throughput: process hundreds of samples per day
• Proven accuracy: achieve unrivaled reproducibility and accuracy with the well-established GoldenGate Assay for Methylation
• Convenient workflow: complete streamlined GoldenGate Assay for VeraCode in two days; reduce manual pipetting steps with pre-kitted beads

VeraCode methylation profiling developed with GoldenGate for Methylation kits allows users to benefit from simplified workflow, assay flexibility, and the data precision made possible by VeraCode technology. VeraScan Software, which comes with the BeadXpress Reader, provides users the flexibility to customize scanning protocols. In addition, our BeadStudio software offers provides a platform for simple to complex data analysis that can be easily exported to third-party data analysis programs.