Whole-Genome DASL assay

The Illumina Human Whole-Genome DASL Assay is an array-based method for expression profiling of partially degraded RNA samples such as those found in FFPE samples.

The Whole-Genome DASL Assay combines the unique PCR and labeling steps of the original DASL Assay with the gene-based hybridization and whole-genome probe set of the HumanRef-8 BeadChip. This BeadChip features content covering more than 24,000 annotated genes derived from RefSeq (Build 36.2, Release 22). The Whole-Genome DASL Assay greatly increases the target set of the original DASL Assay while retaining the ability to accurately profile partially degraded RNA samples.

Whole-Genome DASL Assay Highlights

     

  • High Sensitivity: start with FFPE samples, brain tissue, blood samples without globin reduction, or as few as 100 cells

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  • Low-Sample Input: use 10-100 ng total RNA from fresh-frozen, 50-200 ng from FFPE samples

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  • High Multiplex Capabilities: analyze more than 24,000 annotated probes as once

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  • High-Quality Results: obtain qPCR-equivalent results with high reproducibility (r2>0.86), as well as excellent correlation with matching fresh/frozen samples (r2>0.98)

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  • Low Per-Sample Cost: pay less than one-third the cost of other methods

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  • Whole Genome DASL Assay Kit