Whole-Genome DASL HT assay

The Illumina Human Whole-Genome DASL HT Assay is an array-based method for expression profiling of partially degraded RNA samples such as those found in FFPE samples.

The Whole-Genome DASL HT Assay combines the unique PCR and labeling steps of the original DASL Assay with the gene-based hybridization and whole-genome probe set of our Direct Hibridization Assay. This BeadChip features content covering more than 29,000 annotated genes derived from RefSeq (Build 36.2, Release 38). The Whole-Genome DASL HT Assay greatly increases the target set of the original DASL Assay while retaining the ability to accurately profile partially degraded RNA samples.

Whole-Genome DASL HT Assay Highlights

  • High Sensitivity: start with FFPE samples, brain tissue, blood samples without globin reduction, or as few as 100 cells
  • Low-Sample Input: use 10-100 ng total RNA from fresh-frozen, 50-200 ng from FFPE samples
  • High Multiplex Capabilities: analyze 12 samples per run
  • High-Quality Results: obtain highly reproducible expression profiles (r2>0.97)
  • Low Per-Sample Cost: pay less than one-third the cost of other methods