Quantitative Real-Time PCR (qPCR) has been the technique of choice for gene expression analysis studies for decades. Although effective, current probe-based assays can exhibit cross-reactivity issues, require tedious design procedures, and involve expensive reagents. NuPCR reagents constitute a novel approach that uses a unique NuZyme™ complex for cost-effective, sensitive, and specific qPCR on any Real-Time PCR instrument. In addition, web-based DesignStudio™ software is available to simplify assay design and ordering.
In this webinar, Gothami Padmabandu will discuss NuPCR chemistry and the ease of designing duplexed assays. In addition, experimental design and data analysis using NuPCR reagents in a number of different qPCR applications will be presented.