Functional gene fusions result in over-expression of one portion of the gene and lack of expression in the portion that has been translocated away. This results in an imbalance of expression levels within the gene of interest. Anchored Multiplex
PCR (AMPTM) uses molecular barcodes which enable the counting of unique molecules across a region to assess difference of expression levels. In the CTL panel, select targets are designed to enable the calculation of expression imbalance
as a confirmatory method for detecting gene fusions.